N-acetylcysteine compositions and methods for the treatment and prevention of cysteine/glutathione deficiency in diseases and conditions

ABSTRACT

Life-threatening hepatotoxicity in the setting of acetaminophen overdose is due to depletion of glutathione (GSH), a vital cysteine-containing tripeptide that protects cells and organs against oxidant injury. Rapid administration of N-acetylcysteine (NAC), which provides the cysteine necessary to replenish the depleted GSH, is the standard of care for preventing injury in acetaminophen overdose. Beneficial effects of NAC treatment have also been demonstrated in respiratory, cardiovascular, endocrine and infectious and other diseases. In fact, over fifty randomized placebo-controlled trials conducted in diverse clinical settings document positive responses to NAC treatment. The present invention relates to cysteine/glutathione (GSH) deficiency as a previously unrecognized clinical entity that can complicate the course of commonly encountered diseases and methods of treatment of this generalized deficiency involving administering N-acetylcysteine (NAC) or a pharmaceutically acceptable salt or derivative to a subject in need thereof and monitoring the subjects appropriate glutathione blood levels as needed.

This application claims the benefit of the filing date of provisional applications Ser. No. 60/496,119 and 60/496,127, both of which were filed Aug. 19, 2003.

GOVERNMENT SUPPORT

This work is supported at least in part by grants from the N.I.H. CA42509-14. The government may have certain rights in this invention.

FIELD OF THE INVENTION

The present invention relates to cysteine/glutathione deficiency as a previously unrecognized clinical entity that can complicate the course of commonly encountered diseases and methods of teatment of this generalized deficiency comprising administering N-acetylcysteine or a pharmaceutically acceptable salt or derivative to patients in need thereof and monitoring the appropriate glutathone blood levels as needed.

SUMMARY OF THE INVENTION

The present invention relates to cysteine/glutathione (GSH) deficiency as a previously unrecognized clinical entity that can complicate the course of commonly encountered diseases and methods of treatment of this generalized deficiency comprising administering N-acetylcysteine (NAC) or a pharmaceutically acceptable salt or derivative and monitoring the appropriate glutathione blood levels as needed.

According to one embodiment of the invention, a method of treatment to prevent development of gluathione deficiency as a consequence of disease, a treatment, or a condition comprises administering to a subject at risk of glutathione deficieny as a consequence of disease, a treatment, or a condition a therapeutic amount of NAC or a pharmaceutically acceptable salt or derivative suficient to increase intracellular glutathione levels or decrease oxidative stress and measuring and monitoring the level of glutathione in the blood of patients as needed. According to another embodiment of the invention, a method of treatment to restore glutathione levels comprises administering to subjects in need of glutathone level restoration, as determined by measurement or by a physician, a therapeutic amount of NAC or a pharmaceutically acceptable salt or derivative thereof sufficient to increase intracellular glutathione levels or decrease oxidative stress and monitoring restoration by measuring the level of glutathione in blood as needed. According to another embodiment of the invention, a method of treatment to decrease oxidized glutathione levels elevated as a consequence of disease, a treatment, or a condition comprises administering to a subject suffering from oxidative stress a therapeutic amount of NAC or a pharmaceutically acceptable salt or derivative sufficient to decrease oxidized glutathione levels elevated as a consequence of disease and monitoring the level of oxidized glutathione in blood as needed.

BACKGROUND OF THE INVENTION

GSH is a central component of the oxidative-reductive (redox) apparatus of every cell. One of its key functions is to combine with, and thereby inactivate (detoxify), reactive oxidative intermediates (ROI), other oxidative molecules, and certain drugs, exogenous chemicals and toxins. Because GSH is depleted in these reactions, it must continually be replenished to maintain cell and organ viability and to support normal cellular functions. Drug intoxications resulting in severe GSH depletion, notably acetaminophen overdose, cause extensive hepatic injury if treatment to replenish GSH is not initiated before GSH stores are depleted to below critical protective levels.

Synthesis of GSH requires cysteine, a conditionally essential amino acid that must be obtained from dietary sources or by conversion of dietary methionine via the cystathionase pathway. If the supply of cysteine is adequate, normal GSH levels are maintained. In contrast, if supplies of cysteine are inadequate to maintain GSH homeostasis in the face of increased GSH consumption, GSH depletion occurs.

GSH depletion impacts a wide variety of cellular processes, ranging from DNA synthesis and gene expression to sugar metabolism and lactate production. The pleiotropic activity of this key intracellular molecule, which arose very early in evolution, derives from its participation in the energy economy and the synthetic and catabolic activities of virtually all cells. In higher animals, it also participates in regulating the expression or activity of extracellular molecules, including many of the cytokines and adhesion molecules implicated in inflammatory reactions and other disease processes.

Acute GSH depletion causes severe—often fatal—oxidative and/or alkylation injury. This injury can be prevented (in acetaminophen overdose, for example) by rapid treatment with N-acetylcysteine (NAC), an efficient non-toxic source of cysteine, which is necessary to replenish hepatocellular GSH. Chronic or slowly arising GSH deficiency due to administration of GSH-depleting drugs, or to diseases and conditions that deplete GSH, can be similarly debilitating.

Systematic review of a series of randomized placebo-controlled trials conducted over the last 25 years demonstrates that treatment with NAC provides beneficial effects in a number of respiratory, cardiovascular, endocrine and infectious and other disease settings. Rapid administration of NAC is the standard of care for preventing hepatic injury in acetaminophen overdose. It provides the cysteine necessary to replenish a crucial intracellular tripeptide {tilde over (□)} glutamylcysteinylglycine, commonly known as GSH, which is depleted during detoxification of excessive amounts of acetaminophen. Since orally-administered NAC is rapidly converted by first-pass metabolism to cysteine, it results in replenishment of GSH as well as supplying cysteine for additional metabolic and protein synthetic processes. Thus, the beneficial effects observed following NAC treatment in the trials reviewed here suggest that cysteine/GSH deficiency contributes to the pathophysiology of a wide range of diseases and that avoidance of this deficiency may be important in treating these diseases.

GSH is a central component of the oxidative-reductive (redox) apparatus of every cell. One of its key functions is to combine with, and thereby inactivate (detoxify), reactive oxygen intermediates (ROI), other oxidative molecules, and certain drugs, exogenous chemicals and toxins. Because GSH is depleted in these reactions, it must continually be replenished to maintain cell and organ viability and to support normal cellular functions. Drug intoxications resulting in severe GSH depletion, notably acetaminophen overdose, cause extensive hepatic injury if treatment to replenish GSH is not initiated before GSH stores are depleted to below critical protective levels.

Synthesis of GSH requires cysteine, a conditionally essential amino acid that must be obtained from dietary sources or by conversion of dietary methionine via the cystathionase pathway. If the supply of cysteine is adequate, normal GSH levels are maintained. In contrast, if supplies of cysteine are inadequate to maintain GSH homeostasis in the face of increased GSH consumption, GSH depletion occurs.

GSH depletion impacts a wide variety of cellular processes, ranging from DNA synthesis and gene expression to sugar metabolism and lactate production. The pleiotropic activity of this key intracellular molecule, which arose very early in evolution, derives from its participation in the energy economy and the synthetic and catabolic activities of virtually all cells. In higher animals, it also participates in regulating the expression or activity of extracellular molecules, including many of the cytokines and adhesion molecules implicated in inflammatory reactions and other disease processes.

Acute GSH depletion causes severe—often fatal—oxidative and/or alkylation injury. This injury can be prevented (in acetaminophen overdose, for example) by rapid treatment with NAC, an efficient non-toxic source of cysteine, which is able to replenish hepatocellular GSH. Chronic or slowly arising GSH deficiency due to administration of GSH-depleting drugs, or to diseases and conditions that deplete GSH, can be similarly debilitating[1].

The following discussion provides evidence for the development of cysteine/GSH deficiency in a variety of disease settings and considers the biochemical mechanisms through which this deficiency, and its correction, can impact disease processes.

Search and Inclusion Criteria

The following studies were selected by searching the PubMed database using the Endnote interface. Publications included here describe randomized placebo-controlled NAC trials testing N-acetylcysteine (NAC) for efficacy in a variety of disease settings, were located by a search strategy using the following keywords: placebo AND N-acetylcysteine AND NOT Animal. Abstracts were excluded, as were publications that were not in written in the English language. In addition, studies with fewer than 10 patients and/or unclear endpoints were excluded. All other publications that met the search criteria are included. The included studies are discussed below and summarized in table 1, in which we classify the findings according to whether they reports that NAC treatment results in 1) significant clinical benefit, 2) significant findings whose clinical relevance is unclear, or 3) no significant difference between the control and treatment groups.

Publications describing NAC trials that were not placebo-controlled, or describing observational NAC studies conducted with NAC, were located using the keyword N-acetylcysteine in combination with keywords identifying individual diseases. These trials and studies are not exhaustively reviewed here. Many, but not all, are listed by citation in table 2; some are discussed in later sections. We also discuss, but do not exhaustively review, findings from animal studies and from biochemical and other studies that provide information relevant to disease and GSH depletion mechanisms.

GSH Deficiency and Disease

A role for GSH deficiency in the clinical manifestations of a broad spectrum of diseases and conditions is suggested either by the direct documentation of low GSH levels in these conditions or by the demonstration of significant improvement in patient condition following NAC administration. Over fifty randomized placebo-controlled trials demonstrate beneficial effects of NAC treatment (table 1)[2-62] in diseases and conditions that include systemic inflammatory response syndrome (SIRS), Acute Respiratory Distress Syndrome (ARDS), chronic lung disease (CLD), chronic obstructive pulmonary disease (COPD), neurodegenerative disease, cardiovascular disease, alcoholism, infectious disease (e.g., HIV infection, chronic HCV hepatitis), hepatic and renal failure, diabetes, malnutrition and certain autoimmune diseases.

The mechanisms that underlie the development of GSH deficiency in disease are reasonably well understood, at least in some instances. A wide variety of inflammatory and metabolic stimuli common during active disease increase the production of intracellular oxidants. In addition, neutrophils and other cells present at sites of inflammation release oxidants (reactive oxygen and nitrogen intermediates) that enter other cells and add to the internal oxidant burden. GSH provides the main defense against toxic oxidative intermediates by reducing and thereby inactivating them. However, in so doing, GSH is oxidized to GSH disulfide (GSSG). GSSG is then either rapidly reduced to GSH by GSSG-reductase and NADPH or is excreted from the cell and only in part recovered from the circulation.

Factors that may contribute to GSH deficiency include GSH losses that occur when GSH is enzymatically conjugated to exogenous chemicals (drugs, dietary components and toxins) and excreted from the cell as GSH or acetylcysteine mercapturates (conjugates). In addition, disease processes may decrease the cellular uptake or synthesis of cysteine or cystine, increase GSH efflux[63], or increase the loss of cysteine/GSH sulfur due to accelerated oxidation to the final oxidized forms (sulfate and taurine)[64, 65]. Because a balance between cysteine supply and GSH utilization must be maintained, if oxidant production or levels of substrate for GSH conjugation are high and cysteine supplies for GSH replenishment become limiting, severe GSH deficiency may occur.

Importantly, there are significant potential iatrogenic contributions to GSH depletion. Inadvertent treatment with higher doses of acetaminophen than patients can tolerate is perhaps the most common. This can be particularly dangerous for patients with conditions in which GSH depletion tends to occur as a consequence of the disease process or following treatment with drugs that are detoxified by GSH. In addition, long-term maintenance on parenteral nutrition may result in GSH depletion since parenteral nutrition formulations are not necessarily designed to provide adequate cysteine equivalents to meet the metabolic needs of diseased patients. In the absence of adequate attention to maintenance of adequate cysteine supplies, physicians and other caregivers can inadvertently contribute to GSH deficiency.

Patient behavior may also result in the development of GSH deficiency. Chronic over-consumption of alcohol is well known to deplete GSH in certain tissues, particularly the liver, and thus to render patients susceptible to acetaminophen toxicity at doses well below those that cause toxicity in healthy individuals. Indeed, the FDA has issued a warning to this effect (http://www.fda.gov/ohrms/dockets/ac/O₂/briefing/3882b1.htm). However, chronic consumption of acetaminophen or other GSH-depleting drugs, even well below toxic dose levels, can gradually deplete GSH to the point where these drugs elicit toxicity. Such practices become more dangerous if patients are malnourished or are GSH deficient for other reasons.

In summary, GSH deficiency occurs more frequently than previously suspected. GSH is readily replenished by de novo synthesis as long as sufficient supplies of cysteine are available, either directly from dietary sources or indirectly by conversion of dietary methionine. However, failure to obtain sufficient dietary cysteine to replace that lost when GSH is oxidized or conjugated to drugs or exogenous chemicals results in cysteine, and hence GSH, deficiency (referred to here as cysteine/GSH deficiency) that may necessitate pharmacological intervention.

Dietary Sources of Cysteine

Cysteine utilized in the body is derived from dietary cysteine and methionine, sulfur-containing amino acids that are largely obtained from digested protein. Since mammals obtain cysteine both directly from the diet and by degradation of dietary methionine, the normal cysteine requirement can be satisfied from dietary sources. However, as indicated above, an additional source of cysteine may be required when cysteine loss (e.g., via GSH loss) outstrips the usual dietary supply.

Requirements for sulfur-containing amino acids in humans are based upon nitrogen and sulfur amino acid balance studies conducted with healthy individuals. The average American diet contains about 100 g of protein daily, greater than half of which is animal protein with a relatively high content of sulfur-containing amino acids. The recommended daily allowance (RDA) for sulfur amino acids (SAA) for an adult male is about 1 g (200 mg of methionine and an additional 810 mg methionine that can be replaced by an equivalent amount of cysteine[66]). A healthy, well-fed person will often consume greater than twice the sulfur amino acid RDA. However, poor appetite and/or a tendency to select fresh food with low sulfur amino acid content or bioavailability[67] or processed food depleted of sulfur amino acids[68-70] can result in cysteine deficiency even in otherwise healthy people. Furthermore, as evidence here indicates, the need for sulfur amino acids can be substantially increased in many disease states.

The limited ability of the body to store amino acids is an additional problem. The human liver does contain a reservoir of cysteine (about 1 g) that is largely present in GSH. Since this amount approximates the daily sulfur amino acid requirement, it provides only a short-term source to maintain a stable cysteine supply despite intermittent methionine and cysteine consumption. Under conditions of excessive cysteine requirements or deficient cysteine/methionine consumption, GSH is also released from skeletal muscle and other tissues to supply cysteine. This results in decreased antioxidant and detoxification functions throughout the body. Consequently, even short term inadequate intake of sulfur amino acids can pose a risk to individuals who may consume adequate amounts most of the time[71, 72].

Mechanisms that May Mediate the Clinical Effects of Cysteine/GSH Deficiency

GSH has multiple roles in cells, ranging from neutralization of (ROI) to acting as a co-enzyme in a variety of metabolic processes. The widespread participation of GSH in biochemical reactions of importance to cell growth, differentiation and function offers mechanistic insights into how interfering with GSH homeostasis could influence the course of varied disease processes. A full discussion of the preclinical data bearing on these issues is beyond the present scope. However, to provide a mechanistic context for the clinical findings we discuss, we have summarized some of the key processes regulated by GSH:

Oxidative reactions. In its most well-known role, GSH participates in enzyme mediated reactions to neutralize ROI and thus prevents the accumulation of ROI damage to DNA, proteins and lipids. Glutathione peroxidases play a key role in this process by catalyzing the reaction of GSH with peroxides, including hydrogen peroxide and lipid peroxides. Thus, decreasing GSH can sharply augment oxidative damage and result in cell death or loss of function.

DNA synthesis. Low GSH availability can impair DNA synthesis since GSH acts (via glutaredoxin) as a coenzyme for ribonucleotide reductase, an enzyme required for the synthesis of deoxyribonucleotides[73-75].

Gene expression and signal transduction. GSH has been shown to regulate or influence the expression of several genes, notably inflammatory genes under the control of transcription factors neucular factor kappa B (NF-□B) and activator protein 1 (AP-1), even in settings where there is no marked overproduction of ROI. In addition, GSH has been shown to regulate T cell signaling by controlling phosphorylation of phospholipase C (PLC)□ 1, which is required to stimulate the calcium flux that occurs early in the T cell receptor signaling cascade[76, 77]. Importantly, GSH has also been shown to regulate the expression of vascular cell adhesion molecule-1 (VCAM-1) on vascular endothelial cells, one of the early features in the pathogenesis of atherosclerosis and other inflammatory diseases[21, 78-81].

Enzymes and protein functions. GSH regulates the activity of enzymes and other intracellular molecules by post-translational modifications (glutathionylations) that control the oxidation state of protein-SH groups. When intracellular GSH is at its normal level for a particular cell type in a healthy individual, most of the free protein thiol groups are reduced, i.e., are present as protein-SH. In contrast, when GSH levels are low and/or GSSG levels are increased, GSH is reversibly coupled to many free thiols to create mixed disulfides (protein-S-S-G)[82]. These S-glutathionylated proteins, which may be functionally altered, then persist as such until GSH levels return to normal.

By controlling the activities of a series of enzymes and other intracellular proteins, glutathionylation can rapidly and reversibly alter the metabolic status of cells in response to changes in the redox environment. For example, glutathionylation has been shown to regulate actin polymerization[83], to inhibit the activity of several key enzymes (including glyceraldehyde-3-phosphate dehydrogenase, carbonic anhydrase and protein tyrosine phosphatase) and to activates or stabilize other enzymes (including HIV-1 protease and the NF-□B transcription factor[84]). Nitrosylation of protein thiols has similarly been shown to increase under oxidative conditions[85-88] and to alter functions of key enzymes[89] and other molecules[90-92]. Thus, both glutathionylation and nitrosylation are of central importance to mechanisms through which cysteine/GSH deficiency may impact cell, and hence organ, function.

As indicated above, these types of post-translational modifications are highly sensitive to shifts in the intracellular redox balance. They are rapidly initiated when GSH is depleted and rapidly reversed when GSH is replenished. As such, they provide the kind of flexible response to oxidative stress necessary for organisms living in an oxidative environment. However, at the extreme, they may underlie some of the pathologic changes that occur when chronic cysteine/GSH deficiency occurs in disease.

Glutaredoxin and thioredoxin. Glutaredoxin (Grx) and thioredoxin (Trx) belong to the two major disulfide reductase enzyme families, which take electrons from GSH and NADPH via Grx reductase and Trx reductase, respectively[73-75, 93-95].

Trx and Grx interact with proteins to regulate functional activity, both directly and via glutathionylation. Intracellular GSH and GSSG levels play a major role in this regulation. The activity of Grx is directly regulated by the amount of intracellular GSH and GSSG, which controls the status of the Grx active site. The active sites in Trx (Cys-Gly-Pro-Cys) and Grx (Cys-Pro-Tyr-Cys) contain a dithiol that can be oxidized when GSH levels are low (or GSSG levels increase) to form an internal disulfide between the two cysteine residues or a mixed disulfide in which GSH is bound to one or both cysteine residues in the active site. Formation of the Grx mixed disulfide[82, 93, 96] represents a special case of protein glutathionylation since it arms the Grx for glutathionylation of other proteins. Although Trx can also be glutathionylated[96], current data indicate that glutathionylation is mainly mediated by the Grx mixed disulfide[97-99].

Oxidation of Grx and Trx active sites can also regulate Trx or Grx functions mediated by direct binding to key intracellular proteins. For example, under reducing conditions, Trx and Grx protect cells from apoptosis by binding to and inactivating the apoptosis-signaling kinase I[99, 100] whereas this binding is blocked and apoptosis induction proceeds at low GSH levels (and/or high GSSG levels)[74, 75].

Selenoenzymes. Decreasing GSH increases the intracellular redox potential of the GSH/GSSG couple and puts an additional burden on the thioredoxin-thioredoxin reductase system. This may be quite important in patients who have low selenium levels, since human thioredoxin reductases are selenoenzymes with an essential selenocysteine residue in the active site[75, 101-105]. Cysteine/GSH deficiency in these patients, in whom thioredoxin reductase activity is compromised, may make them particularly susceptible to cell damage under oxidative stress.

Thus, cysteine/GSH deficiency can impact cell and organ function through multiple pathways operating at the same or different sites, depending on the underlying mechanisms responsible for depleting GSH. This potential for affecting different pathways in different diseases perhaps explains why the effects of cysteine/GSH deficiency have not been readily recognizable as a single clinical entity.

N-Acetylcysteine (NAC) Treatment to Relieve Cysteine/GSH Deficiency

Clinical experience in the treatment of acetaminophen toxicity has established that rapid administration of NAC, an essentially non-toxic cysteine source, restores normal GSH levels in solid tissues and the systemic circulation and thus prevents the potentially lethal consequences of severe cysteine/GSH deficiency induced by acetaminophen overdose. In addition to this well-known role for NAC, however, NAC treatment has been shown to be clinically beneficial in a wide variety of diseases and conditions. In fact, over fifty randomized placebo-controlled trials (table 1) have reported beneficial effects of NAC treatment. Collectively, these studies demonstrate that cysteine/GSH deficiency is an important emerging clinical entity and that NAC administration offers an effective method for treating this deficiency.

Although various forms of cysteine and its precursors have been used as nutritional and therapeutic sources of cysteine, NAC is the most widely used and extensively studied*. NAC is about 10 times more stable than cysteine and much more soluble than the stable cysteine disulfide, cystine. L-2-oxothiazolidine-4-carboxylate (procysteine/OTC) has also been used effectively in some studies[106] as have glutathione and glutathione monoethyl ester[107]. In addition, dietary methionine is an effective source of cysteine, as is S-adenosylmethionine (referred to either as SAM or SAM-e)[108]. We focus on NAC in this review because NAC is the cysteine source for used correcting cysteine/GSH deficiency in most studies and because NAC is already approved for therapeutic use for treatment of acetaminophen overdose and as a mucolytic agent in cystic fibrosis.

Surprisingly, given the diverse roles that GSH plays in cellular physiology and regulation of enzyme activity and protein function (see above), GSH deficiency has mainly been discussed from a clinical perspective in terms of the loss of intracellular protection against oxidative stress. Similarly, NAC is principally considered to be an antioxidant rather than a source of cysteine for GSH replenishment. However, while antioxidants such as vitamin E and vitamin C can spare GSH under conditions of oxidative stress, GSH loss due to oxidative or detoxifying reactions can only be offset by GSH resynthesis, which requires a cysteine source.

Cysteine/GSH Deficiency and NAC Therapy in Disease Settings

In the sections that follow, we discuss reported outcomes of NAC therapy in various clinical settings. We largely focus on findings from the randomized placebo-controlled studies (table 1), but also discuss selected findings from observational studies that further illuminate clinical aspects of cysteine/GSH deficiency. Citations for these and other observational NAC treatment studies are presented in table 2, which provides a partial survey of the literature in this area.

Acetaminophen Toxicity.

The toxicity of acetaminophen is due to depletion of GSH in hepatocytes“ ” [109-114]. Acute dose levels of acetaminophen likely to cause severe liver toxicity are well established for healthy individuals[110]. However, under conditions in which GSH levels are compromised, doses of acetaminophen that are within the usual prescribed range can also cause hepatic injury and failure[71, 110]. Thus, acetaminophen usage, and the usage of other GSH-depleting drugs, may be quite important to overall pathology in diseases and conditions such as those discussed in the section that follow, where GSH deficiency is known to occur.

Hepatic and Gastrointestinal Disease.

Hepatic failure (Table 1a). Acetaminophen overdose is a well-known cause of fulminant hepatic failure. In fact, a recent study indicates that acetaminophen overdose and idiosyncratic drug reactions have now replaced viral hepatitis as the most frequent causes of acute liver failure in the United States[115]. N-acetylcysteine (NAC), which provides the cysteine necessary to replenish GSH depleted by toxic agents, is extremely effective in preventing liver damage due to acetaminophen toxicity. Although placebo-controlled studies demonstrating this point are scarse (one report), NAC administered promptly and at a sufficient dose^(e.g.,)[2, 71, 109, 110, 113, 116-159] has been the standard of care for treatment of acetaminophen poisoning for many years[110]. In addition, NAC has been suggested as treatment for Amanita phalloides poisoning[160] and for other exposures to toxic agents^(e.g.,)[161-163].

The importance of administering NAC within the first twenty-four hours of acetaminophen overdose is clear. However, later administration of NAC has also been shown to be effective. In a placebo-controlled study of patients with acetaminophen-induced fulminant hepatic failure who had not previously received NAC, both trial arms showed similar rates of deterioration and recovery of liver function (table 1a). However, subjects in the NAC arm showed a significant increase in survival and a corresponding decrease in the incidence of cerebral edema and hypotension requiring inotropic support [2].

Consistant with these findings, an observational study with one hundred patients who have already developed fulminant hepatic failure due to untreated acetaminophen overdose showed significantly increased survival in the NAC-treated group[138]. A subsequent study, also observational, showed that NAC treatment significantly improves cardiac index, mean oxygen delivery, mean arterial pressure, oxygen consumption and oxygen extraction ratio in patients with fulminant hepatic failure due either to untreated acetaminophen overdose or to other causes[164].

The chronic consumption of alcohol poses a special risk with respect to acetaminophen overdose[139, 147] because alcoholic patients often have lower GSH levels. In such patients, doses of acetaminophen below those usually considered toxic can be expected to deplete GSH below the critical threshold for hepatocellular necrosis[165]. Thus, a recent study suggests that due to an increased risk of developing hepatotoxicity, patients with chronic alcoholism and suspected acetaminophen poisoning should be treated with NAC regardless of risk estimation[166-168].

The US Food and Drug Administration (FDA) recognized the importance of alcohol consumption in predisposing to acetaminophen toxicity by ruling (in 1998) that acetaminophen package labels must include the following warning: “If you consume three or more alcoholic drinks every day, ask your doctor whether you should take acetaminophen or other pain relievers/reducers. Acetaminophen may cause liver damage.” The reasons underlying this ruling are reviewed in a report accessible at <http://www.fda.gov/ohrms/dockets/ac/02/briefing/3882b1 01 E-Final%20Rule.pdf>.

Gastrointestinal disease. Two randomized, placebo-controlled studies showed beneficial effects of NAC treatment on GI-related pathophysiology (see table 1a). In one study conducted as part of an effort to develop chemoprevention for carcinogenesis of the large bowel, NAC treatment was found to lower the proliferative index in the colonic crypt epithelium of subjects who previously had adenomatous polyps [55]. In the second study, NAC treatment in the presence of hyperoxic ventilation better preserved end-organ oxygen utilization and was associated with improved gastric intra-mucosal pH.

A published abstract of a placebo-controlled trial indicated that NAC treatment decreased the sensitivity and specificity of the Helicobacter pylori stool antigen test. However, we were unable to obtain the full text article for evaluation of this conclusion.

Protein-Energy Malnutrition (PEM). Several studies have demonstrated GSH depletion in children with the edematous syndromes of PEM, kwashiorkor and marasmic-kwashiorkor[54, 169-171]. Children with edematous PEM also have elevated levels of biomarkers of cellular oxidant damage[172, 173], indicating that a greater sensitivity to the effects of free radicals on cellular components. The observation that levels of biomarkers of oxidant damage normalize as soon as clinical signs and symptoms resolve[172] suggests that oxidant damage plays an important role in the pathogenesis of the disease.

In a study of children with edematous PEM, Jahoor and colleagues showed that erythrocyte GSH depletion is due to a slower rate of synthesis secondary to inadequate cysteine availability[171]. In another more recent study of a similar group of children with edematous PEM, Jahoor and colleagues demonstrated that GSH synthesis rate and concentration can be restored during the early phase of nutritional rehabilitation if diets are supplemented with NAC[54]. The observation that edema is lost at a faster rate by the group whose GSH pools were restored early with NAC suggests that early restoration of GSH homeostasis in children with edematous PEM accelerates recovery. This possibility is supported by another study showing that increases in GSH levels in children with kwashiorkor are associated with recovery[173].

These findings also raise the question of whether the modest malnutrition common in elderly people, who also frequently have low GSH levels[174], puts the elderly at risk for developing clinically significant cysteine/GSH deficiency and hence at increased risk of hepatic and other tissue injury associated with consumption of GSH-depleting pharmaceuticals such as acetaminophen.

Cardiovascular Disease

Nine placebo-controlled studies showed beneficial effects of NAC treatment in cardiovascular disease (see table 1b). NAC treatment enhanced the coronary vasodilator effects of nitroglycerin in patients with unstable angina, yeilding significant clinical benefit[13, 14, 159, 175-178]. In addition, several placebo-controlled studies demonstrated that treatment with NAC decreased development of nitrate tolerance in patients with stable angina[13, 14, 159, 177]. Nevertheless, the use of NAC in combination with nitroglycerin is limited because it is frequently associated with severe headache[177], most likely due to enhancement of the vasodilator effect.

NAC treatment was also shown to be effective as preventative cardiac measure. A placebo-controlled study evaluating NAC pretreatment in cardiac risk patients examined during periods of hyperoxia showed that the pretreatment attenuated tissue oxygenation impairment and preserved myocardial performance better pretreatment with placebo [11]. NAC treatment in a randomized, placebo-controlled study of 134 chronic hemodialysis patients who are at high risk for cardiovascular events also shows that NAC treatment is associated with a significant decrease (RR 0.60, p=0.03) in composite cardiovascular outcome, including occurrence of myocardial infarction, need for coronary angioplasty or coronary artery bypass surgery, ischemic stroke, or peripheral vascular disease[17]. The NAC-treated group in this study also had much lower serum oxidized LDL values at the end of the study.

In an observational trial with patients undergoing thrombolytic therapy for myocardial ischemia, 24-hour intravenous infusion of GSH significantly decreased the oxidative stress of reperfusion injury as demonstrated by decreased plasma malondialdehyde (MDA) [179]. The GSH-treated patients also had fewer episodes of arrhythmia.

Renal Disease.

Acute renal failure. Nephrotoxicity from acute acetaminophen overdose in the absence of hepatic toxicity has only rarely been described[148, 180]. However, analgesic nephropathy associated with chronic use of compounds containing phenacetin (which is metabolized to acetaminophen) and other non-narcotic analgesic products has been recognized as a cause of renal failure for years[181, 182]. The percentage of incident cases of end-stage kidney failure due to analgesic nephropathy as the principal cause varies from 1% to over 10%, depending on the country considered. In addition, chronic use of these analgesics may contribute to the risk of kidney failure in individuals with chronic kidney injury from other causes. Several large studies have estimated the relative risk of development of kidney failure as a function of analgesic consumption[183, 184]. Most recently, Føred and colleagues reported a dose-dependent increase in the risk of chronic renal failure associated with chronic exposure to acetaminophen in the absence of aspirin use. These investigators showed that the relative risk of chronic renal failure was 5.3 for regular users of acetaminophen consuming ≧1.4 g/day on average[1 84].

Contrast-induced nephropathy (CIN). Six of twelve placebo-controlled studies showed beneficial effects of NAC treatment in CIN (see table 1c). Tepel and colleagues have reported evidence from a placebo-controlled study implicating GSH deficiency in the pathogenesis of contrast nephropathy, a form of acute renal failure[3-5]. These investigators compared NAC to placebo in a study of 83 patients with baseline renal impairment undergoing computed tomography with a nonionic low-osmolality contrast agent. They demonstrated a 90% reduction in the incidence of acute renal failure in subjects treated with NAC, suggesting that GSH replenishment is protective in this clinical setting.

A total of twelve placebo-controlled studies with similar design have been reported[3, 6-10, 185-189]. Five of these[6-10] found about the same level of protection (RR* 0.13 to 0.32) reported by Tepel[3]. Another study, with 183 patients, demonstrated a significant reduction in the incidence of contrast nephropathy (from 8.5% to 0%, p=0.02) only in the subset of patients receiving lower volumes (<140 mL) of contrast agent[190], and yet another[189] showed borderline statistically significant protection for the subgroup of patients with the lowest pre-contrast renal function (creatinine clearances: 30-59 mL/min/1.73 m2). One study [9] addressed the mechanisms by which NAC treatment may protect kidney function and reported increased renal nitric oxide (NO) production in the NAC treated subjects. However, four studies failed to show any protective effect of NAC in preventing contrast nephropathy[185-188]. An additional study using an abbreviated intravenous NAC protocol showed a beneficial effect[191]. *Relative Risk

The striking differences in outcomes (considerable decreases in relative risk vs absence of any protection) in these large, well-designed studies is puzzling, inasmuch as most of the study designs were similar with respect to the degree of renal impairment, percentage of patients with diabetes mellitus, use of saline loading and of nonionic, low-osmolality contrast agents, and the doses of NAC used. The incidence of contrast-induced nephropathy in the placebo-treated groups of the studies that showed protection tended to be higher than in those that did not (23±14% vs 12±6%, P=0.11) although the difference did not achieve statistical significance, suggesting that the difference in outcomes is probably not due predominantly to differences in host factors.

In some negative studies, the follow-up time may have been insufficient to demonstrate the full effects of NAC treatment [189]. In addition, since the source of the NAC used was not stated in most of the studies, it is difficult to exclude differences in NAC potency (related to different sources, vide infra) or the presence of contaminants as a possible factor in the outcomes. Thus, there is currently no obvious explanation for the different findings in these studies. Even so, the majority of the published studies support a strong protective effect of NAC in preventing contrast-induced nephropathy.

Three metaanalyses have recently been published in this highly active area. All found significant heterogeneity among the studies reviewed. Two interpreted the studies to show evidence for reduction in the risk of contrast-induced nephropathy with use of NAC[192, 193]. One concluded that the heterogeneity in the studies precluded aggregation of the data as needed to complete the metaanalysis[194]. Two of the studies recommended initiation of a large randomized placebo-controlled trial to address the efficacy of NAC in this setting[193, 194].

Kidney transplantation Delayed graft function (DGF) after kidney transplantation is probably in large part caused by production of ROI and other reactive species following reperfusion of the transplant organ after a period of warm and cold ischemia. In general, these reactive molecules are detoxified by GSH-dependent mechanisms, including conjugation to GSH by a family of GSH-S-transferase (GST) enzymes, some of which are expressed in large quantity in the proximal tubule of the kidney[195]. In an observational study of 229 kidney transplant recipients, donor (but not recipient) GST MIB polymorphism was associated with significantly lower rates (RR 0.33) of DGF after transplantation[196].

Lack of association with other GST alleles in this study complicates the interpretation of the specific role(s) GST may play in reducing DGF risk. However, since the association was only observed when the protective allele was carried by the transplanted organ, there is reason to suspect that a particular GST (or a closely linked protein) may be protective in this setting.

Endocrine Disease (Insulin Sensitivity)

Three randomized placebo-controlled trials demonstrate beneficial effects of NAC treatment in insulin-related disease (see table 1d). One study demonstrates that oral administration of NAC to patients with non-insulin dependent diabetes mellitus (NIDDM) reverses the elevation of soluble vascular cell adhesion molecule-1 (VCAM-1) [21], a substance which promotes accumulation of macrophages and T-lymphocytes at sites of inflammation and increases progression of vascular damage[79, 80]. A second placebo-controlled study by the same group shows that intravenous GSH infusion significantly increases both intraerythrocytic GSH/GSSG ratio and total glucose uptake in NIDDM patients and suggests that abnormal intracellular GSH redox status in these patients plays an important role in reducing insulin sensitivity[22]. Consistent with these findings, in an ongoing study in type 2 diabetics, Jahoor and colleagues have demonstrated that two weeks of dietary supplementation with NAC elicited significant increases in both erythrocyte GSH concentration and the rate of its synthesis, suggesting that positive clinical effects of NAC are mediated through improved GSH availability[197].

Finally, a study of patients with hyperinsulism secondary to polycystic ovary disease demonstrates that NAC treatment significantly decreases serum insulin levels and insulin sensitivity without altering fasting glucose levels [20]. The authors conclude that NAC may be a new treatment for the improvement of circulating insulin levels and insulin sensitivity in these patients.

Metabolic and Genetic Disease.

Genetic defects that impair GSH synthesis or homeostasis are well known (reviewed by Ristoff[198]). The most common defect affects GSH synthetase (GS) and has a wide range of disease manifestations, including hemolytic anemia, progressive neurological symptoms, metabolic acidosis and, in the most severe form, death during the neonatal period. Data from a small observational study suggests that early supplementation with Vitamins C and E may improve long-term outcome in these patients[199].

Cystic fibrosis. Two placebo-controlled studies report beneficial effects of NAC treatment on lung function in cystic fibrosis (table 1e). A third study reports improvement in measures of lung function, but saw no significant clinical differences between NAC and placebo arm subjects. A very short fourth study (2 weeks) failed to find any significant difference between the trial arms.

Sickle cell disease. In a placebo-controlled trial examining NAC treatment in sickle cell disease, NAC was found to inhibit dense cell formation and decrease the number of episodes of vaso-occlusive disease. These improvements in patient condition were associated with increase in glutathione levels.

Homocysteine metabolism. Two of three NAC randomized, placebo-controlled trials showed beneficial effects of NAC treatment in patients with elevated homocysteine (see table 1e). NAC significantly increased homocysteine removal by hemodialysis. This reduction in plasma homocysteine was significantly correlated with reduction in pulse pressure and improved endothelial function. In a second trial, NAC decreased plasma homocysteine levels in patients with elevated plasma lipoprotein A.

Pulmonary Disease

Chronic lung disease/Chronic Obstructive Pulmonary Disease (CLD/COPD) (see table 1f). In twelve of eighteen randomized, placebo-controlled trials, NAC treated showed beneficial effects for patients with forms of chronic lung disease. Patients to whom NAC was administered orally demonstrated decreased days of illness, improved response to steroids, decreased disease exacerbation rates and a general increase in well-being[23-34]. One study showed a benefit whose clinical relevance is unclear and seven failed to find significant benefit[200-204]. Metaanalyses of the bronchitis studies showed that NAC treatment significantly decreases the costs of hospitalizations, emergency room visits, medications, and work time lost [205-210].

Beneficial effects of NAC treatment have been demonstrated in COPD patients, who showed significant improvements in Forced Expiratory Volume during the first second (FEVI) and Maximum Expiratory Flow (MEF50)[210]. Mucociliary clearance in COPD subjects and in otherwise healthy smokers also showed improvement[36] as did modulation of cancer-associated biomarkers in specific organs in smokers[35];

Systemic Inflammatory Response Syndrome (SIRS) and Acute Lung Injury (ALI); multiple organ system failure (MOSF)(see tables 1f-h). Five of seven randomized placebo-controlled studies showed beneficial effects of NAC as adjunct therapy acute lung injury and end-organ failure. RFesults of these studies indicate that oxidative stress and cysteine/GSH depletion play a major role in inflammation leading to capillary leak syndromes and end-organ failure[44, 46, 211]. These conclusion is supported and partially explained by evidence showing that 1) NAC decreases the cytotoxic effects of TNF-□ and other inflammatory cytokines[212]; 2) NAC decreases neutrophil elastase production in acute lung injury[213-217] and, 3) NAC increases neutrophil protection and decreases mortality in cecal ligation and puncture septic shock[218].

In ALI, results from randomized, placebo controlled trials demonstrate significant antioxidant effects and improved outcome[11, 42, 44, 211, 215, 219-223]. Data from these trials show that NAC treatment decreases the level of respiratory distress, the work of breathing and number of days of mechanical ventilation. In addition, data from the trials show that NAC treatment improves static lung compliance, oxygenation measured either as oxygen index or partial pressure of arterial oxygen (PaO₂), and sustained diaphragmatic muscle activity[211, 219, 222, 223].

Three studies failed to demonstrate significant benefit of NAC treatment in ALI[45, 219, 224], most likely because of the timing of NAC administration. In the studies in which NAC was found to be beneficial [11, 42, 44, 211, 215, 219-223], treatment was initiated 24-48 hours and maintained for 3-10 days after the inciting event. In contrast, in the studies in which no benefit was associated with NAC treatment[45, 219, 224], the treatment was either terminated before day 3 of illness or begun after chronic lung disease with ventilator dependence was well established.

Septic Shock and Infectious Disease.

Septic shock. Three of four randomized placebo-controlled trials demonstrate beneficial effects of NAC treatment in septic shock, as demonstrated by improved tissue oxygenation, decreased veno-arterial PCO₂, decreased IL-8 production, increased cardiac index and overall increased survival. One study, however, demonstrated progressive decrease in mean arterial pressure, cardiac index and stroke work (see table 1g). In this latter study, which is listed as adverse in table 1g, NAC was administered earlier than the preceding studies (two hours after hemodynamic stabilization), which may have confounded the endpoint. In any event, findings from this study suggest that the timing of NAC administration requires further investigation.

HIV disease. A broad series of studies clearly demonstrates GSH levels in erythrocytes, lymphocytes and other peripheral blood mononuclear cells progressively decrease as HIV disease advances[47-49, 225-228]. In addition, careful pharmacokinetic studies demonstrate that the low GSH in HIV-infected individuals is due to limited availability of sufficient cysteine to maintain cellular GSH homeostasis[229, 230]. In fact, a massive peripheral tissue catabolism of sulfur-containing peptides and amino acids has been observed in HIV patients[64, 65].

Five of six randomized placebo-controlled studies show beneficial effects of NAC treatment in HIV infection. Several trials collectively demonstrated that NAC administration to HIV-infected subjects with low GSH levels replenishes lymphocyte and erythrocyte GSH (see table 1 g) [48, 50]. Importantly, one of these studies demonstrates that NAC treatment significantly improves T cell function[50]. This finding supports the idea that cysteine/GSH deficiency contributes to the immunodeficiency in HIV-infected individuals and plays an important and reversible role in the functional impairment of those T cells that are still present at later stages of HIV disease.

Cysteine/GSH deficiency may also contribute to the failure of the innate immune system and the development of opportunistic infections in the final stages of HIV disease. Observational studies have shown that HIV-infected individuals with low CD4 T cell counts and low cellular and systemic GSH levels frequently have elevated blood levels of thioredoxin (Trx), which is an effective chemokine[231]. In mice, circulating Trx (like other chemokines) blocks neutrophil migration to infection sites and hence interferes with innate defense against invading pathogens[218]. Similar interference may occur in HIV infection, since the survival of infected individuals with Trx levels above the normal range is significantly decreased compared to survival of subjects with Trx levels in the normal range[82]. Since NAC treatment lowers Trx levels[94, 232] this may contribute to the observed association between NAC ingestion and prolonged survival in HIV disease[47, 48, 227, 233].

The improvement in T cell function observed in HIV-infected subjects treated with NAC[50] suggests that NAC treatment may be a useful adjunct in HIV vaccination. In addition, this improvement provides a rationale for the strong associations observed between low GSH levels and decreased survival in HIV infection[49] and between NAC administration and improved survival in an open-label NAC study[234].

Influenza. One randomized placebo-controlled trial demonstrated that long-term therapy with oral NAC during “cold” season significantly attenuated the frequency and severity of influenza episodes in elderly subjects and in patients suffering from chronic non-respiratory diseases.

Malaria. NAC (300 mg/kg given intravenously over 20 hours) was tested as adjunctive therapy for severe malaria in two double-blind, placebo-controlled studies[51],[52]. Results from one study (30 subjects[51]) showed that elevated serum lactate levels, an indication of disease severity in potentially life-threatening malaria[235-237], returned to normal twice as quickly in the patients who received NAC than in those who received placebo. Summary of results from a second study (108 subjects [52]) indicate strong positive findings, leading the authors to a call for a large double blind trial of NAC as an adjunctive therapy for severe malaria. (Unfortunately, we were unable to obtain the full text article describing this latter study.)

The mechanism(s) through which NAC produced beneficial effects in these studies were not defined. However, several key processes in falciparum malaria are potential NAC targets. For example, NAC is known to decrease the activity of TNF-□, which has been shown to mediate cerebral dysfunction in murine cerebral malaria[238] and has been implicated in the pathogenesis of severe human malaria[239]. In addition, NAC may act by preventing adherence of Plasmodium falciparum-infected red blood cells to CD36 on postcapillary venular endothelium, an important step in the pathogenesis of severe malaria[240]. Finally, NAC may play a direct role in decreasing serum lactate production by replenishing GSH and thereby reversing the loss of glyceraldehyde phosphate dehydrogenase activity and the shift to glycolytic metabolism likely to occur when GSH is depleted[82].

Multiorgan Failure (table 1h).

NAC treatment for SIRS and MOSF has not been very well studied. One randomized, placebo-controlled study demonstrated that NAC improves ex vivo phagocytosis activity. [220] Three additional studies show a NAC-associated increase in cardiac index and decrease in systemic vascular resistance but have conflicting results with respect to oxygen delivery, oxygen extraction and serum lactate levels [44, 45, 222]. None of these studies report a significant difference in mortality between the NAC and control groups. However, all of the studies were relatively small and had heterogenous SIRS etiologies, suggesting that larger, better-controlled studies are required before conclusions can be drawn with respect to the clinical benefits of NAC treatment in SIRS and MOSF.

The only side effect reported for NAC treatment for inflammatory conditions is mild to moderate anticoagulation[106]. This may be attributable to antioxidant-mediated release of nitric oxide (NO) from sequestered peroxynitrate. It was not found to be clinically significant.

Neurologic and Musculoskeletal Disease (table 1i)

Evidence implicating GSH deficiency in a series of neurogenerative diseases is summarized in a recent review[241] by Schulz and colleagues, who also discuss the use of NAC and other drugs as potential therapeutic approaches. Few randomized placebo-controlled trials have been conducted in this area (table 1i). One, testing NAC treatment in Alzheimer's disease, reported significant benefits for some outcome measures and favorable trends for others[60]. Another, testing NAC treatment in amyotrophic lateral sclerosis, found no significant benefit[242].

NAC treatment was also shown to be beneficial for frail geriatric patients responding to exercise in that it significantly enhanced knee extensor strength and increased the sum of all strength parameters.

Otolaryngolic and Opthamalogic Disease.

Otic disease. Data from a randomized placebo-controlled trial demonstrate that NAC improves response to treatment in otitis media with effusion (OME), a sustained non-specific inflammation of the middle ear mucosa accompanied by secretory transformation of the epithelium and accumulation of fluid in the middle ear space[53] (see table 1j). Instillation of NAC in the middle ear in children who were undergoing their first bilateral insertion of ventilation tubes (VT) due to OME, followed by NAC instillation at day 3 and 7 after VT insertion, significantly decreased OME recurrence, increased the time until VT extrusion (p<0.0167) and decreased VT re-insertions (p<0.025). In addition, the number of episodes of ear problems and visits at the ENT clinic were significantly decreased in the NAC-treated children (p<0.0383).

Pre-clinical studies also point to the importance of oxidative stress and GSH depletion in the genesis of noise and toxin-induced hearing loss[243, 244]. Medications with inner ear toxicity such as aminoglycoside antibiotics and the chemotherapy agent cisplatin have been shown to damage the cochlea through the generation of oxygen free radicals. Hearing loss and cochlear damage associated with administration of these compounds has been shown, in animal models, to be greatly reduced by administration of both NAC and methionine[245-247]. Similarly, studies with animal models show that permanent cochlear damage due to acute acoustic overexposure, which induces ischemia reperfusion, glutamate excitotoxicity, free radical generation and GSH depletion[243, 244, 246, 248], can be almost completely prevented by systemic administration of NAC or methionine[246, 248]. Thus, the stage is now set for clinical trials using NAC or similar agents to prevent or reverse the acute cochlear injury associated with noise or a variety of ototoxins.

Ocular and oral disease. Beneficial effects have been demonstrated following NAC treatment in placebo-controlled studies in chronic blepharitis and in Sjogren's syndrome, where improvements in ocular soreness, ocular irritability, halitosis and daytime thirst were noted. In addition, NAC treatment has been shown to significantly decreas dental plaque formation (table 1j).

Dermatologic Disease (table 1k).

A placebo-controlled trial examining NAC treatment in progressive systemic sclerosis failed to find significant effects of NAC treatment.

Therapeutic Dosing with NAC

NAC is the clinically accepted cysteine source used to treat GSH deficiency due to acetaminophen overdose. It can be administered by intravenous, enteral, and rectal routes. Oral NAC dosages for acetaminophen overdose start with a loading dose of 140 mg/kg body weight followed by doses of 70 mg/kg body weight administered every four hours over a period of three days[129]. Smaller dosages (600 mg-8 g daily) have been administered for substantially longer periods in clinical trials for other conditions (table 1).

Although NAC has been administered orally at quite high dosages, little if any toxicity has been associated with NAC ingestion. The highest reported long-term NAC dosage (an average of 6.9 g/day administered in 3-4 divided doses) was administered to 60 HIV-infected subjects for eight weeks during a placebo-controlled trial and to over 50 subjects for up to six months during the open-label continuation trial[49]. No adverse events requiring physician intervention were observed during either trial segment. During the placebo-controlled segment of the trial, 14/60 subjects reported gastric distress similar to that reported elsewhere[48, 49]. However, half of these subjects (7/14) were in the placebo arm, suggesting that the distress was related to ingestion of the excipient which may have contained significant amounts of lactose.

There are several reports of anaphylactoid and allergic responses in response to intravenous NAC administration[163, 249-256]. These adverse events may in part be explained by findings from preclinical studies demonstrating inflammatory-type responses in animals treated with the oxidized (dimeric) form of NAC[257] which can contaminate NAC preparations that have not been protected against oxidation (see below). In any event, the anaphylactoid reactions to intravenous NAC are easily treated and do not interfere with completion of NAC administration[254].

Finally, caution may be indicated concerning the routine consumption of NAC and other sulfur amino acid precursors in the absence of diseases or conditions leading to cysteine/GSH deficiency, particularly in American and other populations in which the intake animal protein tends to be high and individuals may be ingesting 2-3 times the RDA for sulfur amino acids on a daily basis. We have shown that long-term administration of roughly 6 g/day of NAC to HIV-infected individuals is safe[48, 49]. However, recent observational studies showing that colonic hydrogen sulfide production increases in proportion to consumption of animal protein raises questions[258] as to whether increasing sulfur amino acid intake might predispose toward ulcerative colitis. While the role of hydrogen sulfide in ulcerative colitis is still controversial[259-261], it is important to bear in mind that the long-term risks of routinely increasing sulfur amino acid intake in healthy individuals have not yet been evaluated. Current evidence suggests that 600-900 mg/day, the common daily dosage in Europe for cough and cold relief, may be a reasonable maximum dose for healthy individuals who wish to routinely take NAC.

NAC Formulations

The best known NAC formulation in the US, Mucomyst™ (or the generic version thereof), is available as a 10% or 20% solution of NAC sodium salt that is typically administered orally for treatment of acetaminophen overdose. Since Mucomyst has a strong, disagreeable flavor, it is usually mixed with fruit juice or a soft drink before consumption. Still, as many physicians can attest, patients commonly find it very difficult to tolerate orally, thereby requiring administration via nasogastric tube. Mucomyst is also administered intravenously in some settings, particularly when patients are unconscious or unable to retain the orally administered drug.

To overcome problems with oral administration, European manufacturers produce NAC in pill and capsule formulations. It is also produced and packaged in a variety of effervescent formulations (“fizzy tabs”) that can be dissolved in water, juice or carbonated drinks to create a pleasant tasting, readily tolerated beverage. These formulations are produced under European Good Manufacturing Practice (GMP) standards designed to minimize NAC oxidation to its dimeric form (“di-NAC”), which is pharmacologically active at very low concentrations with immunologic effects opposite to those of NAC[257]. In general, di-NAC constitutes less than 0.1% of the European GMP NAC formulations, which are intended for oral administration and have qualified for health insurance reimbursement[207].

Several US nutraceutical dealers manufacture and sell unbuffered (acidic) NAC. Since the production and packaging of nutraceutical products in the US is not regulated by the FDA, neither the content nor the purity of the NAC formulations currently produced and marketed in the US can be reliably judged. Manufacturing methods for these NAC preparations may not prevent formation of NAC by-products (e.g., di-NAC) and may not have been validated for stability during storage.

We (LAH, JPA, SCD) have recently suggested[262, 263] that NAC be administered, and perhaps co-formulated, with acetaminophen. Animal studies suggest that administration of roughly equimolar amounts of NAC and acetaminophen might be sufficient to accomplish this goal[264]. Co-administration of NAC with a GSH-depleting drug such as acetaminophen could decrease the toxicity of the drug, particular in settings where cysteine/GSH deficiency is likely. Thus, there is strong reason to believe that co-administered NAC would more safely allow administration of acetaminophen or other GSH-depleting drugs when clinically warranted.

Conclusion: Cysteine/GSH Deficiency—a New Clinical Entity

The evidence reviewed here reveals cysteine/GSH deficiency as an emerging clinical entity. The manifestations of this deficiency may vary in different disease settings, as may the biochemical mechanisms that mediate its effects. However, they are united by a common positive response to NAC therapy in over fifty randomized placebo-controlled trials (see table 1). Thus, the studies we have reviewed collectively argue for consideration of cysteine/GSH deficiency as a significant and treatable clinical entity.

Surprisingly, given the diverse roles that GSH plays in cellular physiology and regulation of enzyme activity and protein function, the consequences of low GSH levels have mainly been discussed from a clinical perspective in terms of the loss of protection against intracellular oxidative stress. However, while antioxidants such as vitamin E and vitamin C can spare GSH under conditions of oxidative stress, GSH loss can only be offset by GSH resynthesis, indicating a central role for this molecule over and above its ability to counteract the effects of intracellular oxidants.

Similarly, although NAC is a well-known source of cysteine for GSH replenishment in acetaminophen toxicity, it is principally cast as an antioxidant in other settings. By and large, physicians and the public in general tend to equate NAC with vitamin C, vitamin E and other antioxidants. Indeed, like GSH, NAC can itself serve as an antioxidant. However, while other antioxidants can replace NAC and GSH in this role, only NAC or another cysteine source can provide the raw material necessary to replenish GSH and to enable GSH-dependent biochemical reactions.

We have pointed out that physicians may find NAC administration useful as adjunct therapy for diseases and conditions in which cysteine/GSH deficiency is likely to play a role. The positive findings in the placebo-controlled studies we have discussed support this argument. However, the absence of large multicenter trials testing NAC in various settings leaves this evidence still open to question. Hopefully, the recognition that cysteine/GSH deficiency is an important clinical entity will encourage support for such trials.

In the meantime, a conservative approach to the findings we have discussed suggests that patients with diseases or conditions in which cysteine/GSH deficiency has been demonstrated may be well advised to avoid unnecessary behaviors and exposures to medications that may exacerbate GSH depletion. In fact, when advising such patients, it seems reasonable for physicians to emphasize that alcohol usage be kept at modest levels and that acetaminophen usage should be kept strictly within the recommended dosing (or perhaps be accompanied by NAC administration).

The availability of over-the-counter NAC, and the low toxicity of this cysteine prodrug in situations where it has been tested, opens the possibility of patient or physician initiated therapy. However, if such therapy is elected, we suggest that the NAC preparation(s) used be prepared under GMP conditions and packaged to prevent oxidation of the product.

DETAILED DESCRIPTION OF THE INVENTION

The invention described herein provides a method of treatment to prevent development of gluathione deficiency as a consequence of disease, a treatment, or a condition comprising administering to a subject at risk of glutathione deficieny as a consequence of disease, a treatment or a condition a therapeutic amount of NAC or a pharmaceutically acceptable salt or derivative sufficient to increase intracellular glutathione levels or decrease oxidative stress and measuring and monitoring the level of glutathione in blood in patients as needed. It further provides a method of treatment to restore glutathione levels comprising administering to subjects in need of glutathone level restoration, as determined by measurement or by a physician, a therapeutic amount of NAC or a pharmaceutically acceptable salt or derivative thereof sufficient to increase intracellular glutathione levels or decrease oxidative stress and monitoring restoration by measuring the level of glutathione in blood as needed. It futher provides a method of treatment to decrease oxidized glutathione levels elevated as a consequence of disease, a treatment or a condition comprising administering to a subject suffering from oxidative stress a therapeutic amount of NAC or a pharmaceutically acceptable salt or derivative sufficient to decrease oxidized glutathione levels elevated as a consequence of disease and monitoring the level of oxidized glutathione in blood as needed.

The term “pharmaceutical composition” is used herein to denote a composition that may be administered to a mammalian host, e.g., orally, topically, parenterally, by inhalation spray, or rectally, in unit dosage formulations containing conventional non-toxic carriers, diluents, adjuvants, vehicles and the like. The term “parenteral” as used herein, includes subcutaneous injections, intravenous, intramuscular, intracisternal injection, or by infusion techniques.

The term “therapeutically effective amount” is used herein to denote that amount of a drug or pharmaceutical agent that will elicit the therapeutic response of an animal or human that is being sought.

The pharmaceutical compositions containing NAC may be in a form suitable for oral use, for example, as tablets, troches, lozenges, aqueous, or oily suspensions, dispersible powders or granules, emulsions, hard or soft capsules, or syrups or elixirs. Compositions intended for oral use may be prepared according to any known method, and such compositions may contain one or more agents selected from the group consisting of sweetening agents, flavoring agents, coloring agents, and preserving agents in order to provide pharmaceutically elegant and palatable preparations. Tablets may contain NAC in admixture with non-toxic pharmaceutically-acceptable excipients which are suitable for the manufacture of tablets. These excipients may be for example, inert diluents, such as calcium carbonate, sodium carbonate, lactose, calcium phosphate or sodium phosphate; granulating and disintegrating agents, for example corn starch or alginic acid; binding agents, for example, starch, gelatin or acacia; and lubricating agents, for example magnesium stearate, stearic acid or talc. The tablets may be uncoated or they may be coated by known techniques to delay disintegration and absorption in the gastrointestinal tract and thereby provide a sustained action over a longer period. For example, a time delay material such as glyceryl monostearate or glyceryl distearate may be employed. They may also be coated by the techniques described in U.S. Pat. Nos. 4,356,108; 4,166,452; and 4,265,874, to form osmotic therapeutic tablets for controlled release.

Formulations for oral use may also be presented as hard gelatin capsules where NAC is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin, or a soft gelatin capsules wherein the active ingredient is mixed with water or an oil medium, for example peanut oil, liquid paraffin, or olive oil.

Aqueous suspensions may contain the active compounds in admixture with excipients suitable for the manufacture of aqueous suspensions. Such excipients are suspending agents, for example sodium carboxymethylcellulose, methylcellulose, hydroxypropylmethylcellulose, sodium alginate, polyvinylpyrrolidone, gum tragacanth and gum acacia; dispersing or wetting agents may be a naturally-occurring phosphatide such as lecithin, or condensation products of an alkylene oxide with fatty acids, for example polyoxyethylene stearate, or condensation products of ethylene oxide with long chain aliphatic alcohols, for example, heptadecaethyl-eneoxycetanol, or condensation products of ethylene oxide with partial esters derived from fatty acids and a hexitol such as polyoxyethylene sorbitol monooleate, or condensation products of ethylene oxide with partial esters derived from fatty acids and hexitol anhydrides, for example polyethylene sorbitan monooleate. The aqueous suspensions may also contain one or more coloring agents, one or more flavoring agents, and one or more sweetening agents, such as sucrose or saccharin.

Oily suspensions may be formulated by suspending NAC in a vegetable oil, for example arachis oil, olive oil, sesame oil or coconut oil, or in a mineral oil such as a liquid paraffin. The oily suspensions may contain a thickening agent, for example beeswax, hard paraffin or cetyl alchol. Sweetening agents such as those set forth above, and flavoring agents may be added to provide a palatable oral preparation. These compositions may be preserved by the addition of an anti-oxidant such as ascorbic acid.

Dispersible powders and granules suitable for preparation of an aqueous suspension by the addition of water provide NAC in admixture with a dispersing or wetting agent, suspending agent and one or more preservatives. Suitable dispersing or wetting agents and suspending agents are exemplified by those already mentioned above. Additional excipients, for example, sweetening, flavoring, and coloring agents may also be present.

The pharmaceutical compositions of the invention may also be in the form of oil-in-water emulsions. The oily phase may be a vegetable oil, for example, olive oil or arachis oil, or a mineral oil, for example a liquid paraffin, or a mixture thereof. Suitable emulsifying agents may be naturally-occurring gums, for example gum acacia or gum tragacanth, naturally-occurring phosphatides, for example soy bean, lecithin, and esters or partial esters derived from fatty acids and hexitol anhydrides, for example sorbitan monooleate, and condensation products of said partial esters with ethylene oxide, for example polyoxyethylene sorbitan monooleate. The emulsions may also contain sweetening and flavoring agents.

Syrups and elixirs may be formulated with sweetening agents, for example glycerol, propylene glycol, sorbitol or sucrose. Such formulations may also contain a demulcent, a preservative and flavoring and coloring agents. The pharmaceutical compositions may be in the form of a sterile injectible aqueous or oleaginous suspension. This suspension may be formulated according to the known methods using suitable dispersing or wetting agents and suspending agents described above. The sterile injectable preparation may also be a sterile injectable solution or suspension in a non-toxic parenterally-acceptable diluent or solvent, for example as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution, and isotonic sodium chloride solution. In addition, sterile, fixed oils are conveniently employed as solvent or suspending medium. For this purpose, any bland fixed oil may be employed using synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid find use in the preparation of injectables.

The compositions may also be in the form of suppositories for rectal administration of the compounds of the invention. These compositions can be prepared by mixing NAC alone or in combination with a therapeutically effective amount of a therapeutic agent with a suitable non-irritating excipient which is solid at ordinary temperatures but liquid at the rectal temperature and will thus melt in the rectum to release the drug. Such materials include cocoa butter and polyethylene glycols, for example.

For topical use, creams, ointments, jellies, solutions of suspensions, etc., containing the compounds of the invention are contemplated. For the purpose of this application, topical applications shall include mouth washes and gargles.

The NAC alone or in combination with a therapeutically effective amount of a therapeutic agent may also be administered in the form of liposome delivery systems, such as small unilamellar vesicles, large unilamellar vesicles, and multilamellar vesicles. Liposomes may be formed from a variety of phospholipids, such as cholesterol, stearylamine, or phosphatidylcholines.

Pharmaceutically-acceptable salts of NAC alone or in combination with a therapeutically effective amount of a therapeutic agent, where a basic or acidic group is present in the structure, are also included within the scope of the invention. The term “pharmaceutically acceptable salts” refers to non-toxic salts of NAC alone or in combination with a therapeutically effective amount of a therapeutic agent, which are generally prepared by reacting the free base with a suitable organic or inorganic acid or by reacting the acid with a suitable organic or inorganic base. Representative salts include the following salts: Acetate, Benzenesulfonate, Benzoate, Bicarbonate, Bisulfate, Bitartrate, Borate, Bromide, Calcium Edetate, Camsylate, Carbonate, Chloride, Clavulanate, Citrate, Dihydrochloride, Edetate, Edisylate, Estolate, Esylate, Fumarate, Gluceptate, Gluconate, Glutamate, Glycollylarsanilate, Hexylresorcinate, Hydrabamine, Hydrobromide, Hydrocloride, Hydroxynaphthoate, Iodide, Isethionate, Lactate, Lactobionate, Laurate, Malate, Maleate, Mandelate, Methanesulfonate, Methylbromide, Methylnitrate, Methylsulfate, Monopotassium Maleate, Mucate, Napsylate, Nitrate, N-methylglucamine, Oxalate, Pamoate (Embonate), Palmitate, Pantothenate, Phosphate/diphosphate, Polygalacturonate, Potassium, Salicylate, Sodium, Stearate, Subacetate, Succinate, Tannate, Tartrate, Teoclate, Tosylate, Triethiodide, Trimethylammonium and Valerate. When an acidic substituent is present, such as-COOH, there can be formed the ammonium, morpholinium, sodium, potassium, barium, calcium salt, and the like, for use as the dosage form. When a basic group is present, such as amino or a basic heteroaryl radical, such as pyridyl, an acidic salt, such as hydrochloride, hydrobromide, phosphate, sulfate, trifluoroacetate, trichloroacetate, acetate, oxlate, maleate, pyruvate, malonate, succinate, citrate, tartarate, fumarate, mandelate, benzoate, cinnamate, methanesulfonate, ethanesulfonate, picrate and the like, and include acids related to the pharmaceutically-acceptable salts listed in the Journal of Pharmaceutical Science, 66, 2 (I 977) p. 1-19.

Other salts which are not pharmaceutically acceptable may be useful in the preparation of NAC alone or in combination with a therapeutically effective amount of a therapeutic agent and these form a further aspect of the invention.

Thus, in another aspect of the present invention, there is provided a pharmaceutical composition comprising NAC alone or in combination with a therapeutically effective amount of a therapeutic agent, or a pharmaceutically acceptable salt, solvate, or prodrug therof, and one or more pharmaceutically acceptable carriers, excipients, or diluents.

In another aspect of the present invention, there is provided a pharmaceutical composition comprising a therapeutically effective amount of NAC alone or in combination with a therapeutically effective amount of a therapeutic agent, or a pharmaceutically acceptable salt, solvate, or prodrug therof, and one or more pharmaceutically acceptable carriers, excipients, or diluents, wherein said therapeutically effective amount comprises a sufficient amount NAC for at least partial amelioration of cysteine/glutathione deficiency in a disease or condition. In an embodiment of the pharmaceutical composition, the disease comprises chronic obstructive pulmonary disease (COPD). In another embodiment of the pharmaceutical composition, the disease comprises acute renal failure. In another embodiment of the pharmaceutical composition, the disease comprises sickle cell anemia. In another embodiment of the pharmaceutical composition, the disease comprises comprises diabetes mellitus. In another embodiment of the pharmaceutical composition, the disease comprises inflammatory diseases. In another embodiment of the pharmaceutical composition, the disease comprises human immunodeficiency virus mediated disease. In another embodiment of the pharmaceutical composition, the disease comprises malaria. In another embodiment of the pharmaceutical composition, the disease comprises protein-energy malnutrition. In another embodiment of the pharmaceutical composition, the disease comprises otic disease. In another embodiment of the pharmaceutical composition, the disease comprises neurodegenerative disease. In another embodiment of the pharmaceutical composition, the disease comprises cardiovascular disease.

In another aspect, the present invention provides a pharmaceutical composition comprising a therapeutically effective amount of NAC alone or in combination with a therapeutically effective amount of a therapeutic agent, and one or more pharmaceutically acceptable carriers, excipients, or diluents, wherein the pharmaceutical composition is used to replace or supplement compounds that restore cysteine/glutathione levels.

In another aspect, the present invention provides a method for improvement of cysteine/glutathione deficiency in a disease or condition comprising administering to a subject in need thereof a NAC alone or in combination with a therapeutically effective amount of a therapeutic agent, wherein NAC alone or in combination with a therapeutically effective amount of a therapeutic agent is administered to the subject as a pharmaceutical composition comprising a therapeutically effective amount of NAC and one or more pharmaceutically acceptable carriers, excipients, or diluents, wherein said therapeutically effective amount of NAC comprises a sufficient amount of NAC for treatment or prevention of cysteine-glutathione deficiency in the disease or condition.

The term “treatment” as used herein, refers to the full spectrum of treatments for a given disorder from which the patient is suffering, including alleviation of one, most of all symptoms resulting from that disorder, to an outright cure for the particular disorder or prevention of the onset of the disorder.

Generally speaking, the amount of NAC per dosage unit is preferably from 1 mg to 25000 mg, preferably at least 3 mg to 2000 mg per dosage unit for oral administration, and 20-20000 mg for parenteral administration. The unit dose of NAC alone or in combination with a therapeutically effective amount of a therapeutic agent, will usually comprise at least about 1.5 mg/kg to a maximum amount of 70 mg/kg (for pediatric doses), usually at least about 500 mg (for adult doses), and usually not more than about 2000 mg at the physician's discretion. Patients on therapy known to deplete cysteine/glutathione or produce oxidative stress may benefit from higher amounts of NAC. The amount of active ingredient that may be combined with the carrier materials to produce a single dosage will vary depending upon the host treated and the particular mode of administration.

This dosage has to be individualized by the clinician based on the specific clinical condition of the subject being treated. Thus, it will be understood that the specific dosage level for any particular patient will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of administration, rate of excretion, drug combination and the severity of the particular disease undergoing therapy.

While the invention has been described and illustrated with reference to certain preferred embodiments therof, those skilled in the art will appreciate that various changes, modifications and substitutions can be made therein without departing from the spirit and scope of the invention. For example, effective dosages other than the preferred dosages as set forth herein may be applicable as a consequence of variations in the responsiveness of the mammal being treated for cysteine/glutathione deficiency in a disease or condition. Likewise, the specific pharmacological responses observed may vary according to and depending on the particular active compound selected or whether there are present pharmaceutical carriers, as well as the type of formulation and mode of administration employed, and such expected variations or differences in the results are contemplated in accordance with the objects and practices of the present invention.

CITATIONS

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Seema, Kumar R, Mandal R N, Tandon A, Randhawa V S, Mehta G,     Batra S, Ray G N, Kapoor A K: Serum TNF-alpha and free radical     scavengers in neonatal septicemia. Indian J Pediatr 1999,     66(4):511-516. -   447. van Bakel M M, Printzen G, Wermuth B, Wiesmann U N: Antioxidant     and thyroid hormone status in selenium-deficient phenylketonuric and     hyperphenylalaninemic patients. Am J Clin Nutr 2000, 72(4):976-981. -   448. Krenzelok E P: New developments in the therapy of     intoxications. Toxicol Lett 2002, 127(1-3):299-305. -   449. Brok J, Buckley N, Gluud C: Interventions for paracetamol     (acetaminophen) overdoses. Cochrane Database Syst Rev     2002(3):CD003328. -   450. Listed N A: N-acetylcysteine. Altern Med Rev 2000,     5(5):467-471. -   451. Sochman J: N-acetylcysteine in acute cardiology: 10 years     later: what do we know and what would we like to know? J Am Coll     Cardiol 2002, 39(9): 1422-1428. -   452. Bains J S, Shaw C A: Neurodegenerative disorders in humans: the     role of glutathione in oxidative stress-mediated neuronal death.     Brain Research Reviews 1997, 25(3):335-358. -   453. Banaclocha M M: Therapeutic potential of N-acetylcysteine in     age-related mitochondrial neurodegenerative diseases. Med Hypotheses     2001, 56(4):472-477. -   454. Reid M, Jahoor F: Glutathione in disease. Curr Opin Clin Nutr     Metab Care 2001, 4(1):65-71.     Table 1     NAC Treatment in Randomzed Placebo-Controlled Studies

Tables entries all refer to randomized placebo-controlled studies in which NAC, or in some cases another GSH-replenishing drug, were administered. Dosage shown is for NAC unless otherwise indicated. The treatment effect is scored as “Beneficial” when reported outcome(s) differs significantly from the placebo group (p<0.05) for a clinical parameter of importance to patient well-being in the disease under study. If a significant difference between the NAC and placebo group was observed by the clinical relevance of the finding is unclear, the treatment effect is scored as “Significant (? Clin rel)”. Failure to find a significant difference is scored as “Not significant” while significant negative effects of NAC treatment are scored as “Adverse”. TABLE 1a Hepatic and GI pathophysiology NAC use in hepatic and GI Study Treatment pathophysiology n length Dosage effect Acetaminophen Toxicity*. IV NAC 50 21 days 150 mg/kg Beneficial administration to subjects with for 15 min; acetaminophen-induced fulminant 50 mg/kg hepatic failure who had not previously for 4 h; received NAC (late presentation) 100 mg/kg increased survival and decreased both over 16 h. the incidence of cerebral edema and the frequency of hypotension requiring inotropic support. Rates of deterioration and recovery of liver function were not affected. Keays, 1991[2] Hepatic transplant patients. NAC 50 5 hours 150 mg/kg Beneficial induces mild vasodilation, improves for 15 min; oxygen delivery and consumption, and 12.5 mg/kg reduces base deficit. Bromley for 4 h. 1995[19] Hepatitis C. Treatment with NAC in 147 1 year 1800 mg NAC not addition to conventional therapy with daily with significant interferon--□ did not result in Interferon-□ significant improvement of the treatment group. Grant 2000[265] Colon cancer. NAC lowered the 64 12 weeks 800 mg NAC Beneficial proliferative index in the colonic crypt daily, po epithelium of human volunteers who previously had adenomatous polyps. Estensen, 1999[55] NAC safety. Gastroduodenoscopic 20 7 days 200 mg 3× Safe (no examination following oral NAC daily effect) administration disclosed no lesions. Histological examination of biopsy specimens from the antrum and corpus of the stomach disclosed no pathological changes. Marini, 1980[62] Hyperoxic ventilation. NAC 38 72 hours 150 ml/kg IV Beneficial preserved whole body oxygen uptake, over a period oxygen extraction ratio, and gastric of 15 min intramucosal pH during brief hyperoxic ventilation. Reinhart, 1995[266] Gastric mucosa. The systemic effect 100 3 days 600 mg daily not of NAC on gastric mucus was not prior to significant powerful enough to improve the treatment barium coating of the gastric mucosa. Kinnunen, 1989[267] Helicobacter Pylori. NAC decreased 107 5 ml of 107 Interferes the sensitivity and specificity of the 4% NAC with Helicobacter pylori stool antigen test. TID for traditional Demiturk, 2003[268] 3 days clinical assay Protein-energy malnutrition. 32 50 days 0.5 mmol per Beneficial Malnourished children admitted for kg per day treatment of infection were treated with NAC or placebo (alanine). NAC restored GSH synthesis rate and concentration when administered during the early phase of treatment Badaloo, 2002[54] *Note: Although NAC is the accepted treatment for acetaminophen overdose, there do not appear to be any placebo-controlled trials that supported its initial acceptance. A series of studies that are not placebo-controlled, e.g., Smilkstein et al¹²⁷, have served this purpose (see the citations for acetaminophen under Toxic Agents in Table 2).

TABLE 1b NAC use in cardiovascular pathophysiology Study Beneficial n length Dosage effects Hyperoxic ventilation in cardiac risk 30 30 min 150 mg/kg, IV Beneficial patients. NAC helped preserve whole- body oxygen consumption (VO2), oxygen delivery, cardiac index, left ventricular stroke work index and venoarterial carbon dioxide gradient during brief hyperoxia. Clinical signs of myocardial ischemia such as ST-depression did not occur if patients were prophylactically treated with NAC. Spies, 1996[11] Stable angina and nitrate tolerance. 10 24 hours 4800 mg, po Beneficial None of the patients had developed nitrate tolerance at inclusion. NAC in combination with the long-acting nitrate, isosorbide-5- mononitrate (5-ISMN) significantly prolonged the total exercise time as compared with placebo/5-ISMN. No further effect was obtained after additional NTG doses. Svendsen, 1989[12] Stable angina and nitrate tolerance. The 7 8 days 2400 mg daily, Beneficial reduction in ST segment depression was po significantly more pronounced during isosorbide dinitrate (ISDN) + NAC as compared with ISDN + placebo. Exercise time and time to angina pectoris were unaffected. NAC augmented the antischemic effects of ISDN as assessed by ECG. Boesgaard, 1991[13] Stable angina and nitrate tolerance. 10 30 hours 2 g IV bolus Beneficial Infusion of high doses of NAC in followed by combination with isosorbide dinitrate 5 g/kg/hr IV (ISDN) affects and partially prevents the development of tolerance to antianginal effects normally observed during infusion with ISDN. Boesgaard, 1992[14] Unstable angina. Combined 46 6 hours 5 g, IV Beneficial administration of nitroglycerin (NTG) and NAC in patients with unstable angina pectoris augments the clinical efficacy of NTG, largely by reducing the incidence of acute myocardial infarction. However, the high incidence of severe hypotension with NTG/NAC suggests that this regimen should be used with some caution. Horowitz, 1988[175, 176] Unstable angina. The combination of 200 4 months 600 mg, po Beneficial nitroglycerin and NAC, associated with conventional medical therapy reduces the occurrence of outcome events. The high incidence of side effects (mainly intolerable headaches) limits the clinical applicability of this therapeutic strategy at least at the dosage used in the present study. Ardissino, 1997[177] Unstable angina and nitrate tolerance. 19 72 hours 10 g q 24 h, not Blood pressure and the response to the IV significant glyceryl trinitrate bolus we note significantly affected by the co- administration of intravenous NAC with intravenous isosorbide trinitrate (ISDN). Weber, 1992[269] Atherosclerosis. Oral L-2-oxothiazolidine- 48 4 hours 4.5 g OTC, po Beneficial 4-carboxylic acid (OTC) improves brachial artery flow-mediated dilation and endothelium-derived nitric oxide action in human atherosclerosis. Vita, 1998[15] Cardiac bypass. The oxidative burst 12 24 hours 100 mg/kg Beneficial response of peripheral neutrophils in the bolus; then patients receiving NAC was significantly of 20 mg/kg lower at all times during bypass. Andersen continuous 1995[16] IV Cardiac Bypass. Data shows that 36 36 hours ? 100 mg/kg NEED cardioplegic arrest initiates the apoptosis followed Paper signal cascade in human left ventricalr car- by infusion diac myocytes. This apoptosis induction 20 mg/kg. can effectively ne prevented by NAC. Fischer, 2004. [270] Myocardial ischemia. 24-hour 96 24 hours GSH, 1800 mg Beneficial intravenous infusion of GSH in addition to IV bolus; thrombolytic therapy significantly 20□g/kg decreased plasma MDA, an oxidative per min for stress indicator that is significantly 24 h. elevated by thrombolytic treatment for acute myocardial infarction. Altomare, 1996[179]

TABLE 1c NAC use in renal pathophysiology Study Beneficial NAC use in renal pathophysiology n length Dosage effects Contrast-induced nephropathy (CIN). 83 2 days 600 mg PO bid Beneficial Prophylactic oral administration of NAC along with hydration in patients with chronic renal insufficiency prevents the reduction in renal function induced by iopromide, a nonionic low-osmolality contrast agent (relative risk, RR 0.10) Tepel, 2000, 2001 and 2003[3-5] CIN. NAC protects patients with 200 3 days 600 mg PO bid Beneficial moderate chronic renal insufficiency from contrast-induced deterioration in renal function after coronary angiographic procedures, with minimal adverse effects (RR 0.32). Kay, 2003[6] CIN. NAC decreased the incidence of 54 2 days 600 mg PO bid Beneficial post-catheterization nephropathy (RR 0.21) in patients with stable chronic renal insufficiency undergoing elective cardiac catheterization. Diaz-Sandoval, 2002[7] CIN. NAC decreased the incidence of 121 7 days 400 mg PO bid Beneficial contrast-induced nephropathy (RR 0.13) for 2 days in patients with stable chronic renal insufficiency undergoing coronary angiography. Shyu, 2002[8] CIN. In patients with serum creatinine > 49 4 days 1000 mg daily Beneficial 1.2 mg/dL (106 μmol/L) undergoing elective coronary angiography, NAC was associated with preservation of creatinine clearance at 24 and 96 hours, which decreased significantly in placebo-treated patients (21% at 24 hours and 18% at 96 hours, both P < O.005). Efarti 2003. [9] CIN. NAC treatment prevented the 43 3 days 600 mg 5 doses Beneficial development of CIN by 72 hours in patients with moderate renal insufficiency (serum creatinine ≧ 1.5 mg/dL) undergoing elective cardiac catheterization (4.8% vs 32.2%, P = 0.046). MacNeil 2003. [10] CIN: NAC is strongly associated with 24 4 days 1200 mg daily Beneficial suppression of oxidative stress-mediated proximal tubular injury. Drager, 2004. [271] CIN. NAC failed to prevent CIN at 24 108 1 day 1200 mg not hours following coronary angiography in significant the group as a whole. The overall incidence was low (4.8%). Kefer et al. [189] CIN. NAC failed to prevent CIN by 48 96 2 days 1500 mg for 4 not hours in patients with moderate chronic doses significant renal insufficiency (serum creatinine > 1.2 mg/dL and creatinine clearance < 50 mL/min) undergoing elective coronary angiography. The incidence of CIN in both groups was low (NAC 8.2%, control 6.4%). Oldemeyer 2003 [188] CIN. In patients with reduced renal 183 2 days 600 mg PO bid not function undergoing angiography and/or significant angioplasty, the amount of contrast agent, but not the administration of prophylactic NAC, was a predictor of renal function deterioration. Brigouri, 2000[190] CIN. NAC failed to protect against 123 2 days 600 mg PO bid not radiocontrast-induced nephropathy in significant patients with baseline renal dysfunction (serum creatinine > 1.6 mg/dL or creatinine clearance < 60 mL/min) undergoing “cardiovascular interventions” using low-osmolality nonionic contrast media. Allaqaband et al, 2002[186] CIN. NAC failed to protect against 79 8 1200 mg 1 hr not radiocontrast-induced nephropathy in prior and 3 hrs significant patients with baseline renal dysfunction after procedure (serum creatinine > 1.7 mg/dL) undergoing cardiac catheterization using low-osmolality nonionic contrast media. Durham, 2002[185] CIN. NAC administration had no 179 2 days 600 mg 4 times not influence on the incidence of CIN at 48 daily significant hours (NAC, 13%; control 12%) after elective cardiac catheterization in patients with a serum creatinine > 1.2 mg/dL or a creatinine clearance < 50 mL/min. Boccalandro 2003. [187] CIN. NAC administration had no 80 1 day 600 mg PO tid not significant effect vs. placebo. significant Goldenberg 2004. [272] Hemodyalysis. Acetylcysteine- 20 5 g in 5% Beneficial dependant increase of homocysteine glucose and removal during a hemodialysis session 150 mg/kg for improves plasma homocysteine nephrophy. concentration, pulse pressure and endothelial function in patients withf end-stage renal failure. Scholze, 2004. [273] Hemodialysis. Treatment of patients 134 1-24 months 600 mg bid Beneficial with NAC reduces composite cardiovascular end points. Median treatment time was 14.5 months. Tepel 2003[17]

TABLE 1d NAC use in endocrine pathophysiology Study Beneficial NAC use in endocrine pathophysiology n length Dosage effects Polycystic Ovary Syndrome. NAC 33 6 weeks 1.8-3 g daily Beneficial decreases circulating insulin levels and depending on decreases insulin sensitivity in patients weight with polycystic ovary syndrome. Fulghesu 2002[20] Diabetes. Oral NAC treatment increased 15 1 month 1200 mg/day, po Beneficial erythrocyte GSH concentrations and M GSH:GSSG ratio. NAC also decreased plasma soluble vascular cell adhesion molecule (VCAM-1), a measure of vascular damage in non-insulin dependent diabetes. De Mattia, 1998[21] Diabetes. IV administration of GSH 20 2 hours GSH, 1.35 Beneficial increased both intra-erythrocytic gm²min⁻¹ IV GSH/GSSG ratio and total glucose uptake in non-insulin dependent diabetic (NIDDM) patients. Significant correlations were found between GSH/GSSG ratio. De Mattia, 1998[22]

TABLE 1e NAC use in metabolic and genetic pathophysiology NAC use in metabolic and genetic Study Beneficial pathophysiology n length Dosage effects Sickle Cell Disease/Vaso-occlusive 21 12 Months 2,400 mg daily Beneficial disease. NAC inhibited dense cell formation, restored glutathione levels toward normal, and decreased vaso- occlusive episodes (0.03 to 0.006 episodes per person-days). Pace 2003[18] Cystic Fibrosis (CF) and primary ciliary 54 3 months 600 mg < 30 kg Beneficial dyskinesia (PCD). No effect was seen in or 800 mg > 30 kg PCD patients. However, but lung function perday, po improved in NAC-treated CF patients in the period when the patients suffer most from lower airway infections. Stafanger, 1988[40] CF. NAC treatment of patients with 52 3 months 600 mg < 30 kg Beneficial chronic pulmonary Pseudomonas or 800 mg > 30 kg to a aeruginosa infection did not significantly per day, po subgroup improve lung function in the overall treatment group. In patients with peak expiratory flow rate (PEFR) below 70% of predicted normal values, NAC significantly increased PEFR, forced vital capacity (FVC) and forced expiratory volume in one second (FEV1) during the treatment period. Stafanger, 1989[41] CF. NAC and Ambroxol improved air 36 12 weeks NAC or Beneficial trapping and FEV1. No other clinical Ambroxol differences were observed between the three 200 mg 3 groups. Ratjen, 1985[274] times daily CF. Although the forced expiratory 21 14 days^(M) 9.5 mg/kg/day not volume in one second (FEV1) improved in po (average) significant a number of NAC-trteated patients, the mean increase was within the range of the intra-individual variability. Gotz, 1980[275] Homocysteine in hemodialysis. NAC 20 1 session Intravenous 5 g Beneficial significantly increased the removal of in 5% glucose homocysteine by hemodialisys. Plasma solution homocysteine levels decreased to 12 +/− 7% (mean +/− SD) of predialysis level in the treatment group compared with 58 +/− 22% in the control group (P < 0.01). The reduction of plasma homocysteine concentration was significantly correlated with a reduction of pulse pressure and improved in endothelial function. Scholze 2004[273] Homocysteine in hemodialysis. Plasma 38 4 weeks 1200 mg BID not homocysteine levels decreased with significant chronic oral NAC therapy (−19%) but did not significantly differ from placebo- treated subjects (−8%, P = 0.07). Friedman, 2003 [276] High plasma homocysteine. In a double 11 14 days 4000 mg daily, Beneficial blind cross over trial in patients with high po plasma Lp(a) (>0.3 g/l), NAC treatment had no effect on plasma lipoprotein(a) levels but significantly reduced plasma homocysteine. Wiklund, 1996[277]

TABLE 1f NAC use in Pulmonary pathophysiology NAC use in Pulmonary Study Beneficial pathophysiology n length Dosage effects Asthma. NAC medication had no effect 25 9 weeks 600 mg Not on any spirometric, lung mechanic or significant gas exchange variable, nor on the frequency of pulmonary symptoms. Bylin 1987[23] Bronchitis. The frequency of 69 6 months 600 mg/day, Beneficial exacerbations was significantly lower in 3 days/wk, po the NAC-treated group. Grassi and Morandini, 1976[24] Bronchitis. Oral NAC changes the 29 4 weeks 600 mg/day, po Beneficial consistency of sputum with resultant ease of expectoration and the expectoration of increased volumes of sputum; peak expiratory flow rate was increased. Aylward, 1980[25] Bronchitis. NAC was effective in 215 10 days 600 mg/day, po Beneficial decreasing sputum volume and viscosity in patients with acute and chronic bronchitis. Brocard, 1980[26] Bronchitis. Oral NAC was associated 744 6 months 400 mg/day, po Beneficial with decreased thickness of sputum, improved ease of expectoration, and decreased incidence of exacerbations. Grassi, 1980, Ferrari, 1980[27, 28] Bronchitis. The exacerbation rate was 259 6 months 400 mg/day, po Beneficial significantly lower in the NAC-treated group. Boman, 1983[29] Bronchitis. Although improvement in 155 3 months 600 mg/day, po Beneficial subjective symptoms (sputum viscosity and character, difficulty in expectoration and cough severity) occurred in both treatment groups, improvements in difficulty in expectoration and cough severity were greater in patients receiving NAC. Jackson, 1984[30] Bronchitis. The number of days on 526 6 months 600 mg/day, po Beneficial which subjects in the NAC group were incapacitated was significantly decreased. There was no statistically significant difference in the number of exacerbations between the NAC and placebo groups although there was a slight trend towards improvement in the NAC group during the first 3 months. Parr and Huitson, 1987[31] Bronchitis. NAC-treated group had 116 6 months 600 mg/day, po Beneficial reduced number of sick-leave days caused by exacerbations of chronic bronchitis. Rasmussen and Glennow, 1988[32] Bronchitis. Pre-treatment with NAC 37 22 weeks 1200 mg/day, po Beneficial resulted in a significantly greater increase in evening peak flows. Evald, 1989[33] Bronchitis. NAC was significantly 153 22 weeks 1200 mg/day, po Beneficial superior to placebo in terms of a favourable effect on General Health Questionnaire score, which measures general well-being in patients with mild chronic bronchitis.. Hansen, 1994[34] Bronchitis. No significant result was 59 2 years 600 mg daily not found with respect to the frequency of significant coughs, dyspenea, sleep disorder, expectorate, morning and afternoon PEF, and PEF, FEV. Heinig, 1985[200] Bronchitis. There was a trend toward 181 5 months 600 mg/day, po not significantly reduced number of significant exacerbations in the NAC-treated group but differences did not reach conventional levels of statistical significance. McGavin, 1985[159] Bronchitis. No significant differences 9 4 weeks 600 mg/day, po not were found in lung function, significant mucociliary clearance curves or sputum viscosity following treatment with NAC compared to control or placebo measurements. Millar, 1985[201] Bronchitis. Differences between the 161 5 months 600 mg/day, po not NAC and placebo groups did not reach significant conventional levels of statistical significance for the number of exacerbations, total days taking an antibiotic; total days spent in bed, number of withdrawals, incidence of side effects, drug compliance, and the patients' assessment of the treatment. McGavin, 1985[202] Bronchitis. NAC delivered by metered 65 16 weeks 4 mg twice not dose inhalers did not have any daily, inhaled significant significant effect on patients' feeling of well-being, sensation of dyspnea, intensity of coughing, mucus production, or expectoration or lung function. NAC effect on exacerbations could not be estimated because the number of reported exacerbations was too low. Dueholm, 1992[204] COPD. Long-term oral administration 44 12 months 600 mg fizzy Beneficial of NAC attenuates hydrogen peroxide formation in the airways of COPD subjects. Kasielski, 2001[210] COPD. The NAC group had 60 10 days 600 mg Beneficial significantly improved mucus viscosity, effective cough and clinical symptoms. Verstraeten, 1979[39] COPD. The major findings of this 9 15 1800 mg for 4 Beneficial study was that short-term, High-dose days and 600 NAC treatment impoved quadriceps mg the day of endurance, reduced the disturbance in the test the prooxidant system and prevented exercise induced oxidative stress. Koechlin, 2004. [278] Smoking. Improved mucus clearance in 12 30 days 600 mg/day Beneficial subjects on NAC when compared to placebo. Olivieri, 1985[36] Smoking. Observed an inhibitory effect 41 6 months 1200 mg/day, po Beneficial of NAC toward the formation of lipophilic-DNA adducts, which can modulate certain cancer-associated biomarkers. VanShooten 2002[35] Bronchopulmonary displasia. 22 24 hours 5% by tracheal Adverse Intratracheal NAC administration administration neither improved clinical condition nor hastened recovery in premature infants with chronic lung disease. Importantly, NAC administration in this form led to increased total airway resistance and cyanotic spells. Bibi, 1992[203] Bronchopulmonary dysplasia (BPD) 391 6 Days 16-32 mg/kg/d not NAC did not prevent BPD or death in signficant infants with extremely low birthweight. Ahola, 2003. [279] Diaphragm dysfunction. 4 3 weeks 150 mg/kg, IV Beneficial Preadministration of IV NAC attenuates the development of diaphragm fatigue in normal subjects breathing against high inspiratory loads. (Relevant to treatment of patients with ventilatory failure secondary to respiratory muscle fatigue.) Travaline, 1997[37] Mucociliary clearance rates. NAC has 161 60 days .6 g oral daily Beneficial a 35% improvement in mucusalary clearance in subjects classified as slow clearers and protects against lung agressors. Todisco, 1985[38] Elective pulmonary surgery. No 38 5 days 600 mg/day, po not benefit was demonstrated either for significant postoperative pulmonary function or in the frequency of atelectasis. Jepsen, 1989[280] Elective upper laparotomy. In patients 131 6 days 1200 mg pre- not subjected to elective upper laparotomy surgery, po; significant given NAC prophylactically, no 600 mg/day significant differences with respect to postsurgery, po postoperative pulmonary function or in the frequency of atelectasis were detected between the NAC and placebo groups. Jepsen, 1989[280] ARDS. Parenteral NAC treatment 16 3 days 190 mg/kg/day, Significant started within 8 h of diagnosis increases IV (? Clin rel) the intracellular GSH in the granulocytes of ARDS patients without decreasing spontaneous oxidant production by these cells. Laurent, 1996[215] ARDS. No improvement could be 66 6 days 150 mg/kg bolus; not demonstrated in the PaO₂/FiO₂ ratio in then 20 mg/kg/hr, significant the study group as compared with the IV control group on any day. Findings indicate that NAC acts as an anticoagulant and perhaps decreases pulmonary fibrin uptake during ARDS. Jepsen, 1992[223] ALI. Mechanical ventilatory support. 61 3 days 40 mg/kg/day, Beneficial IV NAC treatment improved systemic IV oxygenation and reduced the need for ventilatory support in patients presenting with mild-to-moderate acute lung injury. Development of ARDS and mortality were not significantly reduced. Suter, 1994[211] ALI. Pretreatment with NAC prevents 18 12 hours 72 mg/kg bolus, Beneficial lung injury by diminishing elastase 72 mg/kg over 12 activity in ALI The release of hours, IV mediators, especially myeloperoxidase, is not affected. De Backer, 1996[214] ALI. NAC and OTZ both repleted RBC 48 10 days NAC, 70 mg/kg Beneficial GSH. The number of days of acute lung q 8 hr, IV; injury was decreased and there was also OTZ, 63 mg/kg a significant increase in cardiac index in q 8 hr, IV both treatment groups. There was no difference in mortality among groups. Bernard, 1997[222]

TABLE 1g NAC use in septic shock and infectious disease NAC use in septic shock and infectious Study Beneficial disease n length Dosage effects Septic shock. NAC provided a transient 58 2 hrs 150 mg/kg for 15 Beneficial improvement in tissue oxygenation in min, then 12.5 about half of the septic shock patients, as mg/hr over 90 min, indicated by the increase in V02 and IV gastric intramucosal pH and decrease in veno-arterial PC⁰². The NAC responders had a higher survival rate. Spies, 1994[42] Septic shock. Short-term IV infusion of 22 24 hours 150 mg/kg bolus; Beneficial NAC was well-tolerated, improved then 50 mg/kg respiratory function and shortened ICU over 4 hr, IV stay in survivors. Production of IL-8, a potential mediator of septic lung injury, was attenuated. Spapen, 1998[43] Septic shock. After NAC treatment, 60 2 hours 150 mg/kg over 15 Beneficial hepatosplanchnic flow and function min before 12.5 improved. The increase of liver blood mg/kg/hr for 90 flow index was not caused by min redistribution to the hepatosplanchnic area, but by an increase of cardiac index. Rank, 2000[44] Cardiac performance in septic shock. 20 48 hours 150 mg/kg over 15 Adverse Early administration of NAC (within the min; 50 mg/kg over first 2 hours of hemodynamic next 4 hrs; 100 stabilization) resulted in a progressive mg/kg over next 24 decrease in MAP, cardiac index, and left hrs ventricular stroke work index relative both to subject time-zero measurement and to values for the placebo group (p < .01, repeated-measures analysis of variance). Peake, 1996[281] Influenza. Long term treatment with oral 262 6 months 1200 mg/day, po Beneficial NAC during the cold-season appears to significantly attenuate the frequency and severity of influenza or influenza-like episodes in elderly subjects and/or patients suffering from chronic non- respiratory diseases. De Flora, 1997[46] HIV. In subjects with CD4 counts > 45 4 months 800 mg/day, po Beneficial 200/ul, NAC treatment resulted in normalization of plasma cysteine levels and a lower rate of decline in the CD4 count. Akerlund, 1996[47] HIV. Placebo-controlled study 81 8 weeks 3,200-8,000 mg/day, Beneficial established the long-term safety of NAC po administration and demonstrated that NAC treatment replenishes whole blood GSH and T cell GSH in HIV infected individuals. Viral load was not affected. Replenishment was associated with increased survival in the open-label portion of the study. De Rosa, 2000 and Herzenberg, 1997[48, 49] HIV. NAC treatment but resulted in a 69 7 months 0.6-3.6 g depending Beneficial marked improvement in immune on GSH levels, po function. It did not consistently alter viral load. Subjects with and without antiretroviral therapy (n = 40, n = 29, respectively) were included. Breitkreutz, 2000[50] (Also reviewed in Droge 2000[282]) HIV. A significant increase in whole 37 4 weeks OTC, 1500-3000 Beneficial blood GSH was seen after oral mg/day, po administration of L-2-oxothiazolidine-4- carboxylic acid (OTC), a cysteine pro- drug, in the 1,500 mg and the 3,000 mg dose groups. Barditch-Crovo, 1998[283] HIV. Individuals at the beginning of 20 180 days 600 mg/day Beneficial antiretroviral therapy were supplemented with NAC or placebo. In NAC-treated patients, hematocrit remained stable and an increase in CD4 cell count took place earlier than that in the control group. Spada, 2002[227] HIV. In subjects with CD4 counts < 50 10 weeks 800 mg/day po Not 200/ul, NAC did not replenish either significant cysteine or GSH levels in plasma. NAC did not significantly decrease the risk of adverse reactions to trimethoprim- sulphamethoxazole (TMP-SMX) in this study. Akerlund, 1997[284] Malaria. In severe encephalopathy 30 20 hours 300 mg/kg, IV Beneficial secondary to quinine-treated malaria, serum lactate levels normalized twice as quickly after NAC as after placebo. The NAC-treated patients could be switched from intravenous to oral therapy earlier than individuals who received placebo, but the difference was not significant. Watt, 2002[51]

TABLE 1h NAC use in multiorgan failure Study Beneficial NAC use in multiorgan failure n length Dosage effects Hemodynamic effects. NAC treatment 10 45 min 150 mg/kd Significant increased cardiovascular index and (? Clin rel) vasodilation but did not affect oxygen extraction or lactate concentration. Agusti, 1997. [45] Intensive care. There was no statistically 50 3 to 5 days Intravenous not significant difference between the two groups admin of 150 significant regarding outcome as indicated by mortality mg/kg followed and the required days of inotropic support, by 12/mg/kg mechanical ventilation, and intensive care. Molnar, 1998[285]

TABLE 1i NAC use in neurologic and musculoskeletal pathophysiology NAC use in neurologic and musculoskeletal Study Beneficial pathophysiology n length Dosage effects Alzheimer's Disease. Comparison of interval 47 24 weeks 50 mg/kg/day Beneficial change favored NAC treatment as measured by MMSE, the ADL (Activity of Daily Life) scal and other psychometric tests. Adair, 2001[60] Amyotrophic Lateral Sclerosis. Treatment 110 1 year 50 mg/kg daily not of subjects with ALS did not result in a major significant increase in 12 month survival or reduction in disease progression. Louwerse, 1995[242] Exercise. NAC attenuated the decrease in 8 125 mg/kg/hr Beneficial reduced GSH and the increase in oxidized for 15 min, then GSH resulting from intense, intermittent 25 mg/kg/hr exercise. Time to fatigue was unchanged. Medved, 2003[58] Exercise. This is the first demonstration that 125 mg/kg/hr Beneficial NAC infusion during prolonged submaximal for 15 min, exercise sunstantially enhanced performance in then 25 mg/kg/20 well trained individuals. Medved, 2004. [286] min Exercise. In frail geriatric patients responding 44 6 weeks 1800 mg daily Beneficial to physical exercise, NAC enhanced knee extensor strength, increased the sum or all strength parameters and decreased plasma TNF-□. Hauer, 2003[59]

TABLE 1j NAC use in otolaryngolic, opthamalogic and dental pathophysiology NAC use in otolaryngolic, opthamalogic and Study Beneficial dental pathophysiology n length Dosage effects Otitis Media. Instillation of NAC into one ear 75 39 months 0.05 ml Mucomyst Beneficial at the time of bilateral insertion of ventilation solution at 1, 3 tubes (VTs) and on days 3 and 7 afterwards and 7 days decreased recurrence of otitis media with effusion, decreased re-insertion of VTs, and increased time until VT extrusion. The number of episodes of ear problems and visits to the ENT clinic were decreased. Ovesen, 2000[53] Sjogren's syndrome. NAC treatment 26 6 weeks 200 mg daily Beneficial improved ocular soreness (p = 0.004), ocular irritability (p = 0.006), halitosis (p = 0.033) and daytime thirst (p = 0.033). Walters, 1986[56] Chronic blepharitis. NAC improves tear film 40 4 months 300 mg/day, po Beneficial quality as detected by fluorescein break-up time (FBUT) and mucous fern pattern. Yalcin, 2002[57] Prevention of plaque formation. Comparison 28 2 weeks 10% aqueous Beneficial of plaque indices for control and test periods solution of showed a statistically significant (p < .001) NAC buffered 25.56% reduction on plaque attributable to to pH 6.5 with NAC. Bowles, 1985[61] flavoring agents added

TABLE 1k NAC use in dermatologic pathophysiology NAC use in dermatologic Study Beneficial pathophysiology n length Dosage effects Progressive Systemic Sclerosis. 22 I year 1000- not Several simple measures of 2000 mg significant function and skin distensibility daily were tested. No significant differences between the NAC and placebo groups were observed over the year of the study. Furst, 1979[287]

TABLE 2 NAC treatment: reports of observational studies Clinical findings Classi- Clinical GSH plus GSH fication Disease findings measures measures Hepatic Acetaminophen [2, 71, [128, 133, Function Toxicity 110, 134, 136, 122, 124, 159, 289] 126, 131, 135, 137, 138, 140, 141, 146- 148, 152, 153, 288] Alcohol [290] [168] [291, 292] Hepatitis [161, 293, [295- 294] 299] Renal Chronic [300] [301] Function Kidney Failure Dialysis [302] Nephrotoxicity [3, 190] □-Amanintin [160] Cardio- Angina vascular Arteriosclerosis/ [303- [308] [307, 309] Cardiac Risk 307] Myocardial [310] Infarction Cardiomyopathy [311] Endocrine Diabetes [312- [106, 315- 314] 323] Pulmonary Reviews [324- [327, [329] 326] 328] Bronchopulmonary [330, 331] ARDS [332] [333, 334] Fibrosing [335, 336] Aveolitis Chronic Asthma [23, 337] Chronic [338- Bronchitis/ 340] COPD Cystic [341] [178, 342- Fibrosis 346] Pulmonary [347, [349- Fibrosis 348] 352] Smoking [35] [36, 353- 356] Lung Cancer [357] Critical Intensive [358- Care Care 360] Sepsis/ [42, [43, 364, Septic Shock 361- 365] 363] Malnutrition [173, [54, 170, 366] 171] Infection HIV [367- [370- [379- 369] 378] 388] Helicobacter [389] [390] pylori Influenza [391] Malaria Epilepsy [392, [394, 395] 393] Gastro- Inflammatory [396] [397- intestinal Bowel 399] Disease Barrett's [400] Esophagus Liver Disease [401- [143] [406, 407] 405] Liver [81] transplantation Colon Cancer [408- 411] Optic Blepharitis Cataract [412- 414] Eale's Disease [415] Skin Psoriasis [416] [417] Photodermatosis [418] Immune Rheumatoid [419- System Arthritis 421] Comm. Variable [422] Immunodeficiency Urogenital Prostate [423- [426, 425] 427] Urinary Aging [428- [431- 430] 436] Toxic Arsenic [437] Agents Poisoning Other [438] [439- Chemicals and 441] Medications Perinatal Preeclampsia [442, 443] Neonates [444- 446] Metabolism Phenylketonuria [447] Misc. [448- [241, 452- Reviews 451] 454] Cancer Treatment Review 

1. A method of treatment to prevent development of glutathione deficiency as a consequence of disease, a treatment or a condition comprising administering to a subject at risk of glutathione deficiency as a consequence of disease, or a treatment, or a condition, a therapeutic amount of N-acetylcysteine or a pharmaceutically acceptable salt or derivative thereof sufficient to increase intracellular glutathione levels or decrease oxidative stress and measuring and monitoring the level of glutathone in blood in patients as needed.
 2. A method of treatment to restore glutathione levels comprising administering to subjects in need of glutathione level restoration as determined by measurement or by physician a therapeutic amount of N-acetylcysteine or a pharmaceutically acceptable salt or derivative thereof sufficient to increase intracellular glutathione levels or decrease oxidative stress and monitoring restoration by measuring the level of glutathione in blood as needed.
 3. A method of treatment to decrease oxidized glutathione levels elevated as a consequence of disease, a treatment or a condition, comprising administering to a subject suffering from oxidative stress a therapeutic amount of N-acetylcysteine or a pharmaceutically acceptable salt or derivative thereof sufficient to decrease oxidized glutathione levels elevated as a consequence of disease, a treatment, or a condition and monitoring the level of oxidized glutathione in blood as needed. 